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• W2047759541 abstract "The synthesis of Nerve Growth Factor (NGF) in vitro by tissues of the mouse, rat, and embryonic chick has been investigated by culturing NGF-sensitive nervous tissue (dorsal root ganglia from 8-day chick embryos) adjacent to explants of the tissues tested, and measuring any resulting stimulation of nerve fibre outgrowth. Most of the tissues examined from the mouse and rat caused a stimulation of fibre outgrowth at one or more stages of development, and the fibre outgrowth was inhibited in every case by monospecific antisera to purified mouse NGF. The effect was therefore attributable specifically to NGF. No such stimulation of fibre outgrowth was prompted by a number of chick tissues. The mouse and rat tissues contained no detectable NGF prior to culture, and no evidence was obtained for the release by enzymic action during culture of NGF activity from preexisting “precursor” molecules in either the tissues or the culture medium. No immunoreactive precursors for NGF were detected in either tissue or culture medium prior to culture. No endogenous inhibitors of NGF could be detected in freshly dissected tissues or in the culture medium, and so the results could not be due to the destruction of inhibitors and the consequent revealing of endogenous NGF. It may therefore be concluded that the observed stimulation of nerve fibre outgrowth was due to the de novo synthesis of NGF in vitro. This probably occurs as a response to the culture conditions and should not be interpreted as an indication of NGF synthesis by these tissues in vivo." @default.
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• W2047759541 date "1980-06-01" @default.
• W2047759541 modified "2023-09-27" @default.
• W2047759541 title "The production of Nerve Growth Factor in vitro by tissues of the mouse, rat, and embryonic chick" @default.
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• W2047759541 doi "https://doi.org/10.1016/0012-1606(80)90482-0" @default.
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