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- W2047826369 abstract "The determination of genetic expression has now become necessary for the diagnosis and treatment of patients. Fluorescence in situ hybridization is regarded as the gold standard for some applications, such as HER-2 expression. This method is not very practical for the routine histopathological laboratory. Chromogenic in situ hybridization (i.e., CISH) is presented as the new method and demonstrates the most promise for easy adaptability in most clinical pathology laboratories. This method enables the detection of gene expression in the nucleus using the conventional histochemical reaction. Technologists have become highly skilled in immunohistochemical techniques, and there are similarities in some aspects of the procedures between in situ hybridization and immunohistochemistry. Formalin-fixed tissue usually is pretreated and hybridized with labeled probes. in situ hybridization is the technique currently used to confirm the immunochemical response especially concerning prognostic treatment. With the advent of gene therapy, this technique is becoming in as much demand as traditional immunohistochemistry. (The J Histotechnol 28229, 2005)Submitted October 10, 2005; accepted with revisions December 6, 2005" @default.
- W2047826369 created "2016-06-24" @default.
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- W2047826369 date "2005-12-01" @default.
- W2047826369 modified "2023-09-25" @default.
- W2047826369 title "An Introduction to Chromogenicin situHybridization" @default.
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- W2047826369 doi "https://doi.org/10.1179/his.2005.28.4.229" @default.
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