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- W2047841471 abstract "We have synthesized and examined a number of fully and partly self-complementary palindromic oligodeoxynucleotides for their ability to assume in solution a unimolecular hairpin structure. The main results obtained by a combined optical and electrophoresis investigation show that: (i) DNA folding needs not be driven by mismatched base pairings over the dyad; fully self-complementary palindromatic duplexes, comprising regular (CG)n DNA fragments, possess a considerable intrinsic propensity to make intramolecular base pairings; (ii) The duplex-hairpin interconversion is, in general, a slow process independent of the length and base composition of the palindrome; (iii) The palindromic sequences energetically least favored to form hairpin structures consist of C:G base pairs around the dyad axis and of T:A blocks in the arms of the inverted repeat; (IV) The base composition of the stem strongly influences the hairpin thermal stability. For instance, the substitution of one C:G with one A:T base pair in the stem helix of d(CG)7 diminishes the stability of the hairpin by 9°C. It is found that the stability of the stem helix, in hairpins of defined sequence and with the same loop length, decreases in the order alternating-CG > homo-CG > AC(GT) > alternating-AT, i.e as in polynucleotides. The thermodynaic parameters for the hairpin-coil transition are reported." @default.
- W2047841471 created "2016-06-24" @default.
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- W2047841471 date "1989-07-01" @default.
- W2047841471 modified "2023-10-11" @default.
- W2047841471 title "Hairpin structures in synthetic oligodeoxynucleotides: sequence effects on the duplex-to-hairpin transition" @default.
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- W2047841471 doi "https://doi.org/10.1016/0300-9084(89)90042-4" @default.
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