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- W2047858495 abstract "ATP concentrations in Pinus banksiana Lamb. cuttings were monitored during propagation. The cuttings, which initiate adventitious roots only in basal stem callus, were prepared from 90-day-old seedlings and treated with the auxin N-phenyl indolyl-3-butyramide in 100% ethanol to promote rooting; controls were treated with 100% ethanol alone. The base (lowest 1 em stem), stem (2 em section), and needles (mid-stem) of cuttings were sampled at day 0 and daily for the first 10 days of propagation, before and during root primordium initiation. ATP was measured by bioluminescent assay. Regardless of treatment, ATP concentrations decreased about 25% in stem during the test and were unchanged in needles. In contrast, ATP concentrations in the base of control cuttings about doubled between days 0 and 4, then decreased by day 10 to the day 0 value. Maximum (day 4) ATP concentrations in the base of auxin-treated cuttings were about twice as great as in controls. However, temporal ATP concentrations in the base of control and auxin-treated cuttings were positively related. Overall, spatiotemporal changes in ATP concentrations were positively related to normal and auxin-induced callusing-rooting." @default.
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- W2047858495 date "1966-12-01" @default.
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- W2047858495 title "Extraction, separation, and quantitative estimation of soluble nucleotides and sugar phosphates in plant tissues" @default.
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- W2047858495 doi "https://doi.org/10.1016/0003-2697(66)90188-6" @default.
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