Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047899898> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2047899898 endingPage "114" @default.
- W2047899898 startingPage "106" @default.
- W2047899898 abstract "Long-chain acyl-CoA hydrolase (EC 3.1.2.2), which is found primarily in the brain in rats, catalyzes the hydrolysis of fatty acyl-CoA thioesters. We purified this enzyme, referred to as ACH, from the rat brain cytosol. The molecular masses of the native enzyme and the subunit were estimated to be 104 and 36 kDa, respectively. The enzyme showed high activity with long-chain acyl-CoAs, e.g., with maximal velocity of 262 mumol/min/mg and Km of 5.7 microM for palmitoyl-CoA, but acyl-CoAs with carbon chain lengths of C8-18 were also good substrates. The enzyme was refractory to the inhibitory effect of diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, but sensitive to p-chloromercuribenzoate. In the rat brain cytosol, about 90% of palmitoyl-CoA hydrolase activity was titrated by anti-ACH antibody, which accounted for over 70% of the enzyme activity found in the brain tissue. Immunoblots of the cytosol prepared from rat brain regional blocks indicated the broad distribution of ACH over the brain, with a relatively high level in the pons and medulla. Immunohistochemically, ACH was localized to neurons. In addition to various nuclei, some neuronal cells, such as mitral cells in the olfactory bulb, pyramidal cells in the cerebral cortex, and Purkinje cells in the cerebellum, were also immunostained with anti-ACH antibody. Brain cytosols prepared from ten mammalian species including human contained a single polypeptide reactive to anti-ACH antibody with molecular masses of 34-36 kDa, together with high activities of palmitoyl-CoA hydrolase. These findings suggest the physiological significance of ACH in the brain, although its precise role remains to be determined." @default.
- W2047899898 created "2016-06-24" @default.
- W2047899898 creator A5028454859 @default.
- W2047899898 creator A5033450914 @default.
- W2047899898 creator A5037875387 @default.
- W2047899898 creator A5047192619 @default.
- W2047899898 creator A5085922927 @default.
- W2047899898 date "1996-02-01" @default.
- W2047899898 modified "2023-09-24" @default.
- W2047899898 title "Long-Chain Acyl-CoA Hydrolase from Rat Brain Cytosol: Purification, Characterization, and Immunohistochemical Localization" @default.
- W2047899898 doi "https://doi.org/10.1006/abbi.1996.0053" @default.
- W2047899898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8579357" @default.
- W2047899898 hasPublicationYear "1996" @default.
- W2047899898 type Work @default.
- W2047899898 sameAs 2047899898 @default.
- W2047899898 citedByCount "42" @default.
- W2047899898 countsByYear W20478998982013 @default.
- W2047899898 countsByYear W20478998982014 @default.
- W2047899898 countsByYear W20478998982015 @default.
- W2047899898 countsByYear W20478998982016 @default.
- W2047899898 countsByYear W20478998982017 @default.
- W2047899898 countsByYear W20478998982018 @default.
- W2047899898 countsByYear W20478998982019 @default.
- W2047899898 countsByYear W20478998982020 @default.
- W2047899898 countsByYear W20478998982022 @default.
- W2047899898 crossrefType "journal-article" @default.
- W2047899898 hasAuthorship W2047899898A5028454859 @default.
- W2047899898 hasAuthorship W2047899898A5033450914 @default.
- W2047899898 hasAuthorship W2047899898A5037875387 @default.
- W2047899898 hasAuthorship W2047899898A5047192619 @default.
- W2047899898 hasAuthorship W2047899898A5085922927 @default.
- W2047899898 hasConcept C134018914 @default.
- W2047899898 hasConcept C153911025 @default.
- W2047899898 hasConcept C181199279 @default.
- W2047899898 hasConcept C185592680 @default.
- W2047899898 hasConcept C2778816929 @default.
- W2047899898 hasConcept C2779652256 @default.
- W2047899898 hasConcept C2780496858 @default.
- W2047899898 hasConcept C529278444 @default.
- W2047899898 hasConcept C55493867 @default.
- W2047899898 hasConcept C86803240 @default.
- W2047899898 hasConcept C98539663 @default.
- W2047899898 hasConceptScore W2047899898C134018914 @default.
- W2047899898 hasConceptScore W2047899898C153911025 @default.
- W2047899898 hasConceptScore W2047899898C181199279 @default.
- W2047899898 hasConceptScore W2047899898C185592680 @default.
- W2047899898 hasConceptScore W2047899898C2778816929 @default.
- W2047899898 hasConceptScore W2047899898C2779652256 @default.
- W2047899898 hasConceptScore W2047899898C2780496858 @default.
- W2047899898 hasConceptScore W2047899898C529278444 @default.
- W2047899898 hasConceptScore W2047899898C55493867 @default.
- W2047899898 hasConceptScore W2047899898C86803240 @default.
- W2047899898 hasConceptScore W2047899898C98539663 @default.
- W2047899898 hasIssue "1" @default.
- W2047899898 hasLocation W20478998981 @default.
- W2047899898 hasLocation W20478998982 @default.
- W2047899898 hasOpenAccess W2047899898 @default.
- W2047899898 hasPrimaryLocation W20478998981 @default.
- W2047899898 hasRelatedWork W1563004741 @default.
- W2047899898 hasRelatedWork W1986393148 @default.
- W2047899898 hasRelatedWork W1986592235 @default.
- W2047899898 hasRelatedWork W2004231513 @default.
- W2047899898 hasRelatedWork W2004293894 @default.
- W2047899898 hasRelatedWork W2004754196 @default.
- W2047899898 hasRelatedWork W2030492692 @default.
- W2047899898 hasRelatedWork W2049661544 @default.
- W2047899898 hasRelatedWork W2060038873 @default.
- W2047899898 hasRelatedWork W2083360052 @default.
- W2047899898 hasVolume "326" @default.
- W2047899898 isParatext "false" @default.
- W2047899898 isRetracted "false" @default.
- W2047899898 magId "2047899898" @default.
- W2047899898 workType "article" @default.