Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047949062> ?p ?o ?g. }
- W2047949062 endingPage "2661" @default.
- W2047949062 startingPage "2653" @default.
- W2047949062 abstract "Abstract The phagocyte nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) oxidase was functionally reconstituted in monkey kidney COS-7 cells by transfection of essential subunits, gp91phox, p22phox, p47phox, and p67phox. COS-7 cells express the essential small guanosine 5′-triphosphatase, Rac1. Transgenic COS-phox cells were capable of arachidonic acid–induced NADPH oxidase activity up to 80% of that of human neutrophils, and of phorbol myristate acetate (PMA)–induced activity up to 20% of that of neutrophils. Expression of all 4 phox components was required for enzyme activity, and enzyme activation was associated with membrane translocation of p47phox, p67phox, and Rac1. Expression of p47phox Ser303Ala/Ser304Ala or Ser379Ala phosphorylation-deficient mutants resulted in significantly impaired NAPDH oxidase activity, compared with expression of wild-type p47phox or the p47phox Ser303Glu/Ser304Glu phosphorylation mimic, suggesting that p47phoxphosphorylation contributes to enzyme activity in the COS system, as is the case in neutrophils. Hence, COS-phox cells should be useful as a new whole-cell model that is both capable of high-level superoxide production and readily amenable to genetic manipulation for investigation of NADPH oxidase function. PMA-elicited superoxide production in COS-phox cells was regulated by activation of protein kinase C (PKC) and Rac. Although COS-7 cells differ from human neutrophils in PKC isoform expression, transient expression of major neutrophil isoforms in COS-phox cells did not increase PMA-induced superoxide production, suggesting that endogenous isoforms were not rate limiting. Val204 in p67phox, previously shown to be required for NADPH oxidase activity under cell-free conditions, was found to be essential for superoxide production by intact COS-phox cells, on the basis of transfection studies using a p67phox(Val204Ala) mutant." @default.
- W2047949062 created "2016-06-24" @default.
- W2047949062 creator A5015426313 @default.
- W2047949062 creator A5016536314 @default.
- W2047949062 creator A5049024406 @default.
- W2047949062 creator A5059884488 @default.
- W2047949062 creator A5060621536 @default.
- W2047949062 creator A5078712122 @default.
- W2047949062 date "2002-04-15" @default.
- W2047949062 modified "2023-10-16" @default.
- W2047949062 title "Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic system" @default.
- W2047949062 cites W1501041796 @default.
- W2047949062 cites W1515067457 @default.
- W2047949062 cites W1517631297 @default.
- W2047949062 cites W1522267111 @default.
- W2047949062 cites W1540568932 @default.
- W2047949062 cites W1543562266 @default.
- W2047949062 cites W1562828870 @default.
- W2047949062 cites W1568345151 @default.
- W2047949062 cites W1579721654 @default.
- W2047949062 cites W1581364974 @default.
- W2047949062 cites W1586099293 @default.
- W2047949062 cites W1595045594 @default.
- W2047949062 cites W1598564250 @default.
- W2047949062 cites W1661985738 @default.
- W2047949062 cites W1749116795 @default.
- W2047949062 cites W1839240500 @default.
- W2047949062 cites W1859005920 @default.
- W2047949062 cites W1904041648 @default.
- W2047949062 cites W1940122864 @default.
- W2047949062 cites W1968542957 @default.
- W2047949062 cites W1973375708 @default.
- W2047949062 cites W1975544278 @default.
- W2047949062 cites W1978710638 @default.
- W2047949062 cites W1987213083 @default.
- W2047949062 cites W1989764592 @default.
- W2047949062 cites W1992774063 @default.
- W2047949062 cites W1993216532 @default.
- W2047949062 cites W2001322050 @default.
- W2047949062 cites W2002549957 @default.
- W2047949062 cites W2007123049 @default.
- W2047949062 cites W2008210753 @default.
- W2047949062 cites W2010630254 @default.
- W2047949062 cites W2012604352 @default.
- W2047949062 cites W2022942610 @default.
- W2047949062 cites W2024891861 @default.
- W2047949062 cites W2025505655 @default.
- W2047949062 cites W2030481851 @default.
- W2047949062 cites W2030553086 @default.
- W2047949062 cites W2033931111 @default.
- W2047949062 cites W2034453407 @default.
- W2047949062 cites W2049405895 @default.
- W2047949062 cites W2054821808 @default.
- W2047949062 cites W2059048465 @default.
- W2047949062 cites W2075229049 @default.
- W2047949062 cites W2075238900 @default.
- W2047949062 cites W2076075128 @default.
- W2047949062 cites W2084699108 @default.
- W2047949062 cites W2088164656 @default.
- W2047949062 cites W2089887078 @default.
- W2047949062 cites W2091076807 @default.
- W2047949062 cites W2094450883 @default.
- W2047949062 cites W2095835743 @default.
- W2047949062 cites W2097291302 @default.
- W2047949062 cites W2098352212 @default.
- W2047949062 cites W209860576 @default.
- W2047949062 cites W2100488606 @default.
- W2047949062 cites W2108499829 @default.
- W2047949062 cites W2134030137 @default.
- W2047949062 cites W2143908574 @default.
- W2047949062 cites W215195587 @default.
- W2047949062 cites W2155996040 @default.
- W2047949062 cites W2168308945 @default.
- W2047949062 cites W45933742 @default.
- W2047949062 cites W936023199 @default.
- W2047949062 doi "https://doi.org/10.1182/blood.v99.8.2653" @default.
- W2047949062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11929750" @default.
- W2047949062 hasPublicationYear "2002" @default.
- W2047949062 type Work @default.
- W2047949062 sameAs 2047949062 @default.
- W2047949062 citedByCount "116" @default.
- W2047949062 countsByYear W20479490622012 @default.
- W2047949062 countsByYear W20479490622013 @default.
- W2047949062 countsByYear W20479490622014 @default.
- W2047949062 countsByYear W20479490622015 @default.
- W2047949062 countsByYear W20479490622016 @default.
- W2047949062 countsByYear W20479490622017 @default.
- W2047949062 countsByYear W20479490622018 @default.
- W2047949062 countsByYear W20479490622019 @default.
- W2047949062 countsByYear W20479490622020 @default.
- W2047949062 countsByYear W20479490622021 @default.
- W2047949062 countsByYear W20479490622022 @default.
- W2047949062 countsByYear W20479490622023 @default.
- W2047949062 crossrefType "journal-article" @default.
- W2047949062 hasAuthorship W2047949062A5015426313 @default.
- W2047949062 hasAuthorship W2047949062A5016536314 @default.
- W2047949062 hasAuthorship W2047949062A5049024406 @default.
- W2047949062 hasAuthorship W2047949062A5059884488 @default.