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- W2047955904 abstract "Herpes Simplex Virus type-2 (HSV-2) is the main cause of genital herpes infection. Its prevalence is increasing worldwide and varies widely with generally higher rate in developing than developed countries and urban than rural areas. HSV-2 Iranian isolate was propagated in HeLa cell line. The viral genome was extracted by phenol-chloroform and used as template in nested polymerase chain reactions (n-PCR) to amplify gG-2 gene. The amplified gene was cloned into a cloning vector (pTZ57R/T) and transformed into competent E. coli DH5a. The recombinant vector encoding the gene of interest was confirmed by restriction enzyme analysis and sequencing." @default.
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- W2047955904 date "2007-03-01" @default.
- W2047955904 modified "2023-09-25" @default.
- W2047955904 title "Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein G from an Iranian Isolate" @default.
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- W2047955904 doi "https://doi.org/10.3923/pjbs.2007.955.958" @default.
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