Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047992904> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2047992904 endingPage "34" @default.
- W2047992904 startingPage "24" @default.
- W2047992904 abstract "UDP-N-acetylgalactosamine:globoside alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.-) synthesizing Forssman hapten was solubilized from dog spleen microsomes by a combination of Triton X-100 treatment and sonication. The solubilized enzyme was partially purified by calcium phosphate gel, ammonium sulfate fractionation and then DEAE-cellulose column chromatography. The enzymatic activity of the purified preparation was stimulated by exogenously added phosphatidylserine, as found in the particulate enzyme. When the properties of the purified enzyme were examined in the presence of exogenous phosphatidylserine, the enzyme had an absolute requirement for Mn2+; this was not substituted by Ca2+ or Mg2+. Apparent Km values for UDP-N-acetylgalactosamine and globoside were 1-10(-5) and 5-10(-4) M, respectively. It had a pH optimum of 6.55 regardless of the presence or absence of exogenous lipids. Since the partially purified enzyme was completely free of uridine diphosphatase which was found in the particulate preparaton, the effect of UDP on the transferase activity could be studied. Thus, UDP inhibited 85% of the activity at a concentration of 1.5 mM. p-Cholormercuribenzoate inhibited over 90% of the activity at 2 mM, indicating the transferase to be SH-enzyme." @default.
- W2047992904 created "2016-06-24" @default.
- W2047992904 creator A5000892889 @default.
- W2047992904 creator A5045217220 @default.
- W2047992904 creator A5084219985 @default.
- W2047992904 date "1977-09-01" @default.
- W2047992904 modified "2023-09-24" @default.
- W2047992904 title "Solubilization and partial characterization of UDP-N-acetylgalactosamine:globosideα-N-acetylgalactosaminyltransferase from dog spleen microsomes" @default.
- W2047992904 cites W1486356174 @default.
- W2047992904 cites W1490587232 @default.
- W2047992904 cites W1498685946 @default.
- W2047992904 cites W1524513539 @default.
- W2047992904 cites W1531089468 @default.
- W2047992904 cites W1540554140 @default.
- W2047992904 cites W1568986672 @default.
- W2047992904 cites W1648238048 @default.
- W2047992904 cites W1671589314 @default.
- W2047992904 cites W1773319440 @default.
- W2047992904 cites W1775749144 @default.
- W2047992904 cites W1936737431 @default.
- W2047992904 cites W1946255258 @default.
- W2047992904 cites W1973640226 @default.
- W2047992904 cites W1991119501 @default.
- W2047992904 cites W2001011933 @default.
- W2047992904 cites W2008512838 @default.
- W2047992904 cites W2017552179 @default.
- W2047992904 cites W2020888012 @default.
- W2047992904 cites W2046006068 @default.
- W2047992904 cites W2060993173 @default.
- W2047992904 cites W2153435210 @default.
- W2047992904 cites W2168526937 @default.
- W2047992904 cites W2175622687 @default.
- W2047992904 cites W4244749349 @default.
- W2047992904 cites W4320801208 @default.
- W2047992904 doi "https://doi.org/10.1016/0005-2744(77)90110-3" @default.
- W2047992904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19076" @default.
- W2047992904 hasPublicationYear "1977" @default.
- W2047992904 type Work @default.
- W2047992904 sameAs 2047992904 @default.
- W2047992904 citedByCount "12" @default.
- W2047992904 crossrefType "journal-article" @default.
- W2047992904 hasAuthorship W2047992904A5000892889 @default.
- W2047992904 hasAuthorship W2047992904A5045217220 @default.
- W2047992904 hasAuthorship W2047992904A5084219985 @default.
- W2047992904 hasConcept C159654299 @default.
- W2047992904 hasConcept C181199279 @default.
- W2047992904 hasConcept C185592680 @default.
- W2047992904 hasConcept C203014093 @default.
- W2047992904 hasConcept C2776376580 @default.
- W2047992904 hasConcept C2778918659 @default.
- W2047992904 hasConcept C2779564620 @default.
- W2047992904 hasConcept C2779637612 @default.
- W2047992904 hasConcept C29144244 @default.
- W2047992904 hasConcept C41625074 @default.
- W2047992904 hasConcept C43617362 @default.
- W2047992904 hasConcept C55493867 @default.
- W2047992904 hasConcept C86803240 @default.
- W2047992904 hasConcept C87644729 @default.
- W2047992904 hasConceptScore W2047992904C159654299 @default.
- W2047992904 hasConceptScore W2047992904C181199279 @default.
- W2047992904 hasConceptScore W2047992904C185592680 @default.
- W2047992904 hasConceptScore W2047992904C203014093 @default.
- W2047992904 hasConceptScore W2047992904C2776376580 @default.
- W2047992904 hasConceptScore W2047992904C2778918659 @default.
- W2047992904 hasConceptScore W2047992904C2779564620 @default.
- W2047992904 hasConceptScore W2047992904C2779637612 @default.
- W2047992904 hasConceptScore W2047992904C29144244 @default.
- W2047992904 hasConceptScore W2047992904C41625074 @default.
- W2047992904 hasConceptScore W2047992904C43617362 @default.
- W2047992904 hasConceptScore W2047992904C55493867 @default.
- W2047992904 hasConceptScore W2047992904C86803240 @default.
- W2047992904 hasConceptScore W2047992904C87644729 @default.
- W2047992904 hasIssue "1" @default.
- W2047992904 hasLocation W20479929041 @default.
- W2047992904 hasLocation W20479929042 @default.
- W2047992904 hasOpenAccess W2047992904 @default.
- W2047992904 hasPrimaryLocation W20479929041 @default.
- W2047992904 hasRelatedWork W1645517174 @default.
- W2047992904 hasRelatedWork W1790834371 @default.
- W2047992904 hasRelatedWork W1967042190 @default.
- W2047992904 hasRelatedWork W1967052443 @default.
- W2047992904 hasRelatedWork W2010372900 @default.
- W2047992904 hasRelatedWork W2042153386 @default.
- W2047992904 hasRelatedWork W2052562559 @default.
- W2047992904 hasRelatedWork W2066300938 @default.
- W2047992904 hasRelatedWork W2071092418 @default.
- W2047992904 hasRelatedWork W2326549699 @default.
- W2047992904 hasVolume "484" @default.
- W2047992904 isParatext "false" @default.
- W2047992904 isRetracted "false" @default.
- W2047992904 magId "2047992904" @default.
- W2047992904 workType "article" @default.