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- W2048006917 abstract "Methanethiosulfonate reagents may be used to introduce virtually unlimited structural modifications in enzymes via reaction with the thiol group of cysteine. The covalent coupling of enantiomerically pure (R) and (S) chiral auxiliary methanethiosulfonate ligands to cysteine mutants of subtilisin Bacillus lentus induces spectacular changes in catalytic activity between diastereomeric enzymes. Amidase and esterase kinetic assays using a low substrate approximation were used to establish kcat/KM values for the chemically modified mutants, and up to 3-fold differences in activity were found between diastereomeric enzymes. Changing the length of the carbon chain linking the phenyl or benzyl oxazolidinone ligand to the mutant N62C by a methylene unit reverses which diastereomeric enzyme is more active. Similarly, changing from a phenyl to benzyl oxazolidinone ligand at S166C reverses which diastereomeric enzyme is more active. Chiral modifications at S166C and L217C give CMMs having both high esterase kcat/KM's and high esterase to amidase ratios with large differences between diastereomeric enzymes." @default.
- W2048006917 created "2016-06-24" @default.
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- W2048006917 date "2000-08-01" @default.
- W2048006917 modified "2023-10-18" @default.
- W2048006917 title "Covalent modification of subtilisin Bacillus lentus Cysteine mutants with enantiomerically pure chiral auxiliaries causes remarkable changes in activity" @default.
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- W2048006917 doi "https://doi.org/10.1016/s0968-0896(00)00121-8" @default.
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