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• W2048009587 abstract "A new electrochemical immunosensor has been developed to detect sulfonamide antibiotic residues in food samples. The immunosensor presented uses immunoreagents specifically developed for the broad recognition of the sulfonamide antibiotic family, a graphite composite electrode (GEC), biofunctionalized magnetic μ-particles and electrochemical nanoprobes prepared by labeling the specific antibodies with CdS nanoparticles (CdSNP). After the immunochemical reaction, the CdSNP are dissolved and the metal ions released are reduced at the electrode and read as in the form of current or charge signal, by the well-known anodic stripping technique. Due to the amplification effect on the amperometric/coulombimetric signal produced by the CdSNP, a high detectability can be reached. Thus, sulfapyridine (SPY), one of the most widely used sulfonamide congeners, can be detected in buffer with an IC50current of 0.20±0.25μgL(-1). The immunosensor has been applied to the analysis of residues of this antibiotic in honey samples. Due to the reported formation of sulfonamide-sugar conjugates in this type of matrix, honey samples are first hydrolyzed in acidic media. The use of magnetic particles minimizes the matrix effect allowing to reach a detectability (LOD, limit of detection) of 0.11μgkg(-1) (current measurements), far below the limits established in some countries for these types of residues in honey samples. Due to the use of magnetic racks, multiple samples can be run simultaneously. The whole analysis process can be performed in around 22min." @default.
• W2048009587 created "2016-06-24" @default.
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• W2048009587 date "2013-05-01" @default.
• W2048009587 modified "2023-10-18" @default.
• W2048009587 title "Development of a Coulombimetric immunosensor based on specific antibodies labeled with CdS nanoparticles for sulfonamide antibiotic residues analysis and its application to honey samples" @default.
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• W2048009587 doi "https://doi.org/10.1016/j.bios.2012.12.017" @default.
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