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• W2048017968 startingPage "28" @default.
• W2048017968 abstract "Summary Current understanding of the mechanisms involved in osseointegration following implantation of a biomaterial has led to adhesion quantification being implemented as an assay of cytocompatibility. Such measurement can be hindered by intra‐sample variation owing to morphological changes associated with the cell cycle. Here we report on a new scanning electron microscopical method for the simultaneous immunogold labelling of cellular focal adhesions and S‐phase nuclei identified by BrdU incorporation. Prior to labelling, cellular membranes are removed by tritonization and antigens of non‐interest blocked by serum incubation. Adhesion plaque–associated vinculin and S‐phase nuclei were both separately labelled with a 1.4 nm gold colloid and visualized by subsequent colloid enhancement via silver deposition. This study is specifically concerned with the effects microgroove topographies have on adhesion formation in S‐phase osteoblasts. By combining backscattered electron (BSE) imaging with secondary electron (SE) imaging it was possible to visualize S‐phase nuclei and the immunogold‐labelled adhesion sites in one energy ‘plane’ and the underlying nanotopography in another. Osteoblast adhesion to these nanotopographies was ascertained by quantification of adhesion complex formation." @default.
• W2048017968 created "2016-06-24" @default.
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• W2048017968 creator A5028618534 @default.
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• W2048017968 creator A5082211352 @default.
• W2048017968 date "2008-07-01" @default.
• W2048017968 modified "2023-10-16" @default.
• W2048017968 title "Focal adhesion interactions with topographical structures: a novel method for immuno-SEM labelling of focal adhesions in S-phase cells" @default.
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• W2048017968 doi "https://doi.org/10.1111/j.1365-2818.2008.02013.x" @default.
• W2048017968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18638187" @default.
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