FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048049130> ?p ?o ?g. }

• W2048049130 endingPage "583" @default.
• W2048049130 startingPage "575" @default.
• W2048049130 abstract "A point mutation, lysine 97 --> isoleucine (K97I), in a surface loop in the beta-sheet protein interleukin 1beta (IL-1beta), exhibits increased levels of inclusion body (IB) formation relative to the wild-type protein (WT) when expressed in Escherichia coli. Despite the common observation that less stable proteins are often found in IBs, K97I is more stable than WT. We examined the folding pathway of the mutant and wild-type proteins at pH 6.5 and 25 degrees C with manual-mixing and stopped-flow optical spectroscopy to determine whether changes in the properties of transiently populated species in vitro correlate with the observation of increased aggregation in vivo. The refolding reactions of the WT and K97I proteins are both described by three exponential processes. Two exponential processes characterize fast events (0.1-1.0 s) in folding while the third exponential process correlates with a slow (70 s) single pathway to and from the native state. The K97I replacement affects the earlier steps in the refolding pathway. Aggregation, absent in the WT refolding reaction, occurs in K97I above a critical protein concentration of 18 microM. This observation is consistent with an initial nucleation step mediating protein aggregation. Stopped-flow kinetic studies of the K97I aggregation process demonstrate that K97I aggregates most rapidly during the earliest refolding times, when unfolded protein conformers remain highly populated and the concentration of folding intermediates is low. Folding and aggregation studies together support a model in which the formation of stable folding intermediates afford protection against further K97I aggregation." @default.
• W2048049130 created "2016-06-24" @default.
• W2048049130 creator A5022636295 @default.
• W2048049130 creator A5028110207 @default.
• W2048049130 creator A5043151535 @default.
• W2048049130 creator A5053680151 @default.
• W2048049130 date "1999-12-28" @default.
• W2048049130 modified "2023-10-14" @default.
• W2048049130 title "Aggregation Events Occur Prior to Stable Intermediate Formation during Refolding of Interleukin 1β" @default.
• W2048049130 cites W1517176127 @default.
• W2048049130 cites W1552217451 @default.
• W2048049130 cites W1607587755 @default.
• W2048049130 cites W1976398996 @default.
• W2048049130 cites W1976917751 @default.
• W2048049130 cites W2001812046 @default.
• W2048049130 cites W2002195659 @default.
• W2048049130 cites W2035057765 @default.
• W2048049130 cites W2086247286 @default.
• W2048049130 cites W2087070363 @default.
• W2048049130 cites W2096601751 @default.
• W2048049130 cites W2151837043 @default.
• W2048049130 doi "https://doi.org/10.1021/bi991518m" @default.
• W2048049130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10642182" @default.
• W2048049130 hasPublicationYear "1999" @default.
• W2048049130 type Work @default.
• W2048049130 sameAs 2048049130 @default.
• W2048049130 citedByCount "71" @default.
• W2048049130 countsByYear W20480491302012 @default.
• W2048049130 countsByYear W20480491302013 @default.
• W2048049130 countsByYear W20480491302014 @default.
• W2048049130 countsByYear W20480491302015 @default.
• W2048049130 countsByYear W20480491302017 @default.
• W2048049130 countsByYear W20480491302019 @default.
• W2048049130 countsByYear W20480491302020 @default.
• W2048049130 countsByYear W20480491302021 @default.
• W2048049130 crossrefType "journal-article" @default.
• W2048049130 hasAuthorship W2048049130A5022636295 @default.
• W2048049130 hasAuthorship W2048049130A5028110207 @default.
• W2048049130 hasAuthorship W2048049130A5043151535 @default.
• W2048049130 hasAuthorship W2048049130A5053680151 @default.
• W2048049130 hasConcept C104317684 @default.
• W2048049130 hasConcept C119599485 @default.
• W2048049130 hasConcept C121332964 @default.
• W2048049130 hasConcept C12554922 @default.
• W2048049130 hasConcept C127413603 @default.
• W2048049130 hasConcept C136238340 @default.
• W2048049130 hasConcept C143065580 @default.
• W2048049130 hasConcept C148898269 @default.
• W2048049130 hasConcept C178790620 @default.
• W2048049130 hasConcept C185592680 @default.
• W2048049130 hasConcept C18705241 @default.
• W2048049130 hasConcept C19472624 @default.
• W2048049130 hasConcept C204328495 @default.
• W2048049130 hasConcept C2776545253 @default.
• W2048049130 hasConcept C32909587 @default.
• W2048049130 hasConcept C47701112 @default.
• W2048049130 hasConcept C55493867 @default.
• W2048049130 hasConcept C62520636 @default.
• W2048049130 hasConcept C8010536 @default.
• W2048049130 hasConcept C86803240 @default.
• W2048049130 hasConceptScore W2048049130C104317684 @default.
• W2048049130 hasConceptScore W2048049130C119599485 @default.
• W2048049130 hasConceptScore W2048049130C121332964 @default.
• W2048049130 hasConceptScore W2048049130C12554922 @default.
• W2048049130 hasConceptScore W2048049130C127413603 @default.
• W2048049130 hasConceptScore W2048049130C136238340 @default.
• W2048049130 hasConceptScore W2048049130C143065580 @default.
• W2048049130 hasConceptScore W2048049130C148898269 @default.
• W2048049130 hasConceptScore W2048049130C178790620 @default.
• W2048049130 hasConceptScore W2048049130C185592680 @default.
• W2048049130 hasConceptScore W2048049130C18705241 @default.
• W2048049130 hasConceptScore W2048049130C19472624 @default.
• W2048049130 hasConceptScore W2048049130C204328495 @default.
• W2048049130 hasConceptScore W2048049130C2776545253 @default.
• W2048049130 hasConceptScore W2048049130C32909587 @default.
• W2048049130 hasConceptScore W2048049130C47701112 @default.
• W2048049130 hasConceptScore W2048049130C55493867 @default.
• W2048049130 hasConceptScore W2048049130C62520636 @default.
• W2048049130 hasConceptScore W2048049130C8010536 @default.
• W2048049130 hasConceptScore W2048049130C86803240 @default.
• W2048049130 hasIssue "3" @default.
• W2048049130 hasLocation W20480491301 @default.
• W2048049130 hasLocation W20480491302 @default.
• W2048049130 hasOpenAccess W2048049130 @default.
• W2048049130 hasPrimaryLocation W20480491301 @default.
• W2048049130 hasRelatedWork W1912080976 @default.
• W2048049130 hasRelatedWork W2023600921 @default.
• W2048049130 hasRelatedWork W2055827177 @default.
• W2048049130 hasRelatedWork W2113188411 @default.
• W2048049130 hasRelatedWork W2260493136 @default.
• W2048049130 hasRelatedWork W2382178265 @default.
• W2048049130 hasRelatedWork W2939859775 @default.
• W2048049130 hasRelatedWork W2976965029 @default.
• W2048049130 hasRelatedWork W4210423528 @default.
• W2048049130 hasRelatedWork W4252654006 @default.
• W2048049130 hasVolume "39" @default.
• W2048049130 isParatext "false" @default.
• W2048049130 isRetracted "false" @default.

• next