FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048155092> ?p ?o ?g. }

• W2048155092 endingPage "1655" @default.
• W2048155092 startingPage "1655" @default.
• W2048155092 abstract "Early blight of potato (Solanum tuberosum) is caused by Alternaria solani and occurs annually in Michigan. If left uncontrolled, it can result in yield losses exceeding 20% and impact stored potatoes. The disease is commonly managed using succinate dehydrogenase inhibitor (SDHI) fungicides (1). Unfortunately, recent studies have shown that SDHI resistance has increased dramatically over the past 2 years in A. solani populations (1,2). To investigate the occurrence of SDHI resistance in Michigan, potato leaves with early blight symptoms were collected from fields in Montcalm and Ionia counties, MI, in 2012. To obtain A. solani isolates from leaves, small pieces of leaf tissue (5 × 5 mm) were excised from the center of lesions and transferred on to water agar. Plates were incubated at 25°C overnight to allow conidia to germinate. Single germinated A. solani conidia were transferred to potato dextrose agar (PDA) and incubated at 25°C for 7 days. The identity of cultures was confirmed by colony and conidial morphology (3). Nineteen A. solani isolates were obtained and each was screened for sensitivity to the SDHI fungicides boscalid, penthiopyrad, and fluopyram, using a 50 ppm discriminatory dose based on EC 50 values previously determined (2). Mycelial plugs (~5.5 mm) were transferred to amended and non-amended PDA plates that were incubated at 25°C for 7 days. An isolate was considered highly resistant if fungal growth relative to control plates exceeded 50%, moderately resistant if it was between 35 and 50%, and sensitive if it was less than 35% (2). A sensitive A. solani isolate (AS11) from Bonners Ferry, ID, was used as a control in these experiments. Of all isolates tested, 11% were highly resistant to both boscalid and penthiopyrad and 5% were moderately resistant to both fungicides, 21% were moderately resistant to penthiopyrad alone, and the remaining isolates (84 and 68% respectively) were sensitive to the two fungicides. None of the isolates tested were resistant to fluopyram. Recently, two major mutations, H227R in SdhB and H133R in SdhD, have been identified in highly resistant A. solani isolates in Idaho (2). Because the majority of the identified mutations occur near the 3′ end of each subunit, this region was amplified and sequenced using the following primer sets: SdhB (5′-TACTGGTGGAACCAGGAGGAGTA-3′ and 5′-CATACCACTCTAGGTGAAG-3′), SdhC (5′-CCAAATCACCTGGTACGCCTCG-3′ and 5′-TCATCCGAGGAAGGTGTAGTAAAGGCTG-3′), and SdhD (5′-CCGACTCTATTCTCTGCGCCCT-3′ and 5′-CTCGAAAGAGTAGAGGGCAAGACCCA-3′). In this study, all of the isolates that were highly resistant to both boscalid and penthiopyrad were found to contain the H133R mutation in SdhD, which correlated with the strongest resistance phenotype. To our knowledge, this is the first report of resistance to SDHI fungicides in populations of A. solani on potato in Michigan. These data are concerning as they indicate that the highly resistant isolates have already developed cross-resistance between boscalid and penthiopyrad, despite penthiopyrad not yet having regular use in Michigan. Although all of the isolates tested were sensitive to fluopyram, the discovery of isolates resistant to boscalid and penthiopyrad suggests that all SDHI fungicides should be considered at high risk of resistance development in Michigan. References: (1) K. Fairchild et al. Crop Prot. 49:31, 2013. (2) T. Miles et al. Plant Pathol. doi: 10.1111/ppa.12077, 2013. (3) P. Wharton et al. Plant Dis. 96:454, 2012." @default.
• W2048155092 created "2016-06-24" @default.
• W2048155092 creator A5001597602 @default.
• W2048155092 creator A5003176320 @default.
• W2048155092 creator A5018752965 @default.
• W2048155092 creator A5040383377 @default.
• W2048155092 creator A5081007074 @default.
• W2048155092 creator A5082763027 @default.
• W2048155092 date "2013-12-01" @default.
• W2048155092 modified "2023-10-16" @default.
• W2048155092 title "First Report of Boscalid and Penthiopyrad-Resistant Isolates of <i>Alternaria solani</i> Causing Early Blight of Potato in Michigan" @default.
• W2048155092 doi "https://doi.org/10.1094/pdis-03-13-0279-pdn" @default.
• W2048155092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30716825" @default.
• W2048155092 hasPublicationYear "2013" @default.
• W2048155092 type Work @default.
• W2048155092 sameAs 2048155092 @default.
• W2048155092 citedByCount "7" @default.
• W2048155092 countsByYear W20481550922015 @default.
• W2048155092 countsByYear W20481550922016 @default.
• W2048155092 countsByYear W20481550922018 @default.
• W2048155092 countsByYear W20481550922022 @default.
• W2048155092 countsByYear W20481550922023 @default.
• W2048155092 crossrefType "journal-article" @default.
• W2048155092 hasAuthorship W2048155092A5001597602 @default.
• W2048155092 hasAuthorship W2048155092A5003176320 @default.
• W2048155092 hasAuthorship W2048155092A5018752965 @default.
• W2048155092 hasAuthorship W2048155092A5040383377 @default.
• W2048155092 hasAuthorship W2048155092A5081007074 @default.
• W2048155092 hasAuthorship W2048155092A5082763027 @default.
• W2048155092 hasBestOaLocation W20481550921 @default.
• W2048155092 hasConcept C100701293 @default.
• W2048155092 hasConcept C133479454 @default.
• W2048155092 hasConcept C144027150 @default.
• W2048155092 hasConcept C182076605 @default.
• W2048155092 hasConcept C2778138406 @default.
• W2048155092 hasConcept C2778660310 @default.
• W2048155092 hasConcept C2778786769 @default.
• W2048155092 hasConcept C2779771029 @default.
• W2048155092 hasConcept C2993140171 @default.
• W2048155092 hasConcept C43143990 @default.
• W2048155092 hasConcept C523546767 @default.
• W2048155092 hasConcept C54355233 @default.
• W2048155092 hasConcept C62643968 @default.
• W2048155092 hasConcept C74600772 @default.
• W2048155092 hasConcept C86803240 @default.
• W2048155092 hasConceptScore W2048155092C100701293 @default.
• W2048155092 hasConceptScore W2048155092C133479454 @default.
• W2048155092 hasConceptScore W2048155092C144027150 @default.
• W2048155092 hasConceptScore W2048155092C182076605 @default.
• W2048155092 hasConceptScore W2048155092C2778138406 @default.
• W2048155092 hasConceptScore W2048155092C2778660310 @default.
• W2048155092 hasConceptScore W2048155092C2778786769 @default.
• W2048155092 hasConceptScore W2048155092C2779771029 @default.
• W2048155092 hasConceptScore W2048155092C2993140171 @default.
• W2048155092 hasConceptScore W2048155092C43143990 @default.
• W2048155092 hasConceptScore W2048155092C523546767 @default.
• W2048155092 hasConceptScore W2048155092C54355233 @default.
• W2048155092 hasConceptScore W2048155092C62643968 @default.
• W2048155092 hasConceptScore W2048155092C74600772 @default.
• W2048155092 hasConceptScore W2048155092C86803240 @default.
• W2048155092 hasIssue "12" @default.
• W2048155092 hasLocation W20481550921 @default.
• W2048155092 hasLocation W20481550922 @default.
• W2048155092 hasOpenAccess W2048155092 @default.
• W2048155092 hasPrimaryLocation W20481550921 @default.
• W2048155092 hasRelatedWork W1632235512 @default.
• W2048155092 hasRelatedWork W2374507227 @default.
• W2048155092 hasRelatedWork W2415472210 @default.
• W2048155092 hasRelatedWork W2765891095 @default.
• W2048155092 hasRelatedWork W2989588023 @default.
• W2048155092 hasRelatedWork W3139037677 @default.
• W2048155092 hasRelatedWork W4220878641 @default.
• W2048155092 hasRelatedWork W4299318383 @default.
• W2048155092 hasRelatedWork W4377042402 @default.
• W2048155092 hasRelatedWork W4387023758 @default.
• W2048155092 hasVolume "97" @default.
• W2048155092 isParatext "false" @default.
• W2048155092 isRetracted "false" @default.
• W2048155092 magId "2048155092" @default.
• W2048155092 workType "article" @default.