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• W2048163675 endingPage "823" @default.
• W2048163675 startingPage "815" @default.
• W2048163675 abstract "Three novel phosphoramidites, each bearing a phthaloyl-protected aminooxy tail, were prepared and applied in automated oligonucleotide synthesis. After chain assembly, the phthaloyl protection was removed with hydrazinium acetate. Normal succinyl linker turned to be stable under these conditions, and hence the support-bound oligonucleotide could be converted to a pyrene oxime conjugates by reacting with pyrene carbaldehyde or cis-retinal. Standard ammonolytic deprotection then released the deprotected conjugate in solution. Alternatively, the crude aminooxy-tethered oligonucleotide was immobilized to microscopic polymer particles by reacting the aminooxy function with the particle-bound aldehyde or epoxide groups. These immobilized oligonuceotides were shown to serve properly as probes in a mixed phase hybridization assay." @default.
• W2048163675 created "2016-06-24" @default.
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• W2048163675 date "1999-08-27" @default.
• W2048163675 modified "2023-09-25" @default.
• W2048163675 title "Aminooxy Functionalized Oligonucleotides: Preparation, On-Support Derivatization, and Postsynthetic Attachment to Polymer Support" @default.
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• W2048163675 doi "https://doi.org/10.1021/bc990021m" @default.
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