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- W2048294732 abstract "Ultraviolet (UV) excitation for laser-induced native fluorescence (LINF) detection in capillary electrophoresis (CE) offers impressive performance figures of merit when assaying peptides containing tyrosine or tryptophan residues, catecholamines, indolamines, and a number of other classes of analytes with appreciable fluorescence when excited by UV radiation. One of the largest drawbacks of native fluorescence detection schemes in CE-LINF systems has been the expense and the complexity of the lasers required for excitation in the deep UV wavelength range of 200−300 nm. An improved “turnkey” NeCu laser operating at 248.6 nm interfaced to a sheath flow-based CE system obtains a performance similar to that of large frame frequency-doubled Ar ion lasers. The detection limits for serotonin and dopamine (27 nM and 8 μM, respectively, for ∼3-nL injection) are similar to those obtained using a frequency-doubled Ar ion laser at 257 nm (21 nM and 8 μM, respectively). An example of the detection of serotonin-related analytes from a single-cell electropherogram demonstrates the performance of such a system for mass-limited measurements." @default.
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- W2048294732 date "2001-10-12" @default.
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- W2048294732 title "Ultraviolet Native Fluorescence Detection in Capillary Electrophoresis Using a Metal Vapor NeCu Laser" @default.
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- W2048294732 doi "https://doi.org/10.1021/ac010458z" @default.
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