FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048298097> ?p ?o ?g. }

• W2048298097 endingPage "287" @default.
• W2048298097 startingPage "279" @default.
• W2048298097 abstract "The aim of this study was to develop a new cryopreservation technique to maintain the osteoblast viability in frozen iliac bone and to prove cell viability using cell culture techniques. Human iliac cancellous bones were frozen with and without 10% Me(2)SO at -80 degrees C. The tubes were kept in a -80 degrees C freezer for at least 2 days. After the storage period, the frozen bone was thawed by placing the tube in a 37 degrees C water bath. A serial enzymatic digestion technique using 0.2% collagenase was employed to isolate osteoblast-like cells from the bone. The cells that were released were inoculated into tissue culture flasks containing DMEM supplemented with 10% FCS. They were incubated at 37 degrees C in a humidified atmosphere of 95% air and 5% CO(2). Cells of the second passage were plated at a density of 5 x 10(3)cells/cm(2) in a 24-well plate and used for characterization. For characterization, WST-1 assay, determination of alkaline phosphatase, Type I collagen assay, osteocalcin assay, and von Kossa staining were used. The assays were performed at 3, 6, 9, and 12 days after plating the cells. Based on the results of this study, we conclude that the osteoblast-like cells in the frozen bone can survive, only when the bone is frozen with cryoprotectants to prevent injury during freezing and thawing." @default.
• W2048298097 created "2016-06-24" @default.
• W2048298097 creator A5023418784 @default.
• W2048298097 creator A5079889465 @default.
• W2048298097 creator A5090518514 @default.
• W2048298097 date "2002-06-01" @default.
• W2048298097 modified "2023-09-26" @default.
• W2048298097 title "A new bone banking technique to maintain osteoblast viability in frozen human iliac cancellous bone" @default.
• W2048298097 cites W1549796567 @default.
• W2048298097 cites W1991907069 @default.
• W2048298097 cites W1997843759 @default.
• W2048298097 cites W2034833919 @default.
• W2048298097 cites W2043613085 @default.
• W2048298097 cites W2064788267 @default.
• W2048298097 cites W2068596665 @default.
• W2048298097 cites W2076732638 @default.
• W2048298097 cites W2130339953 @default.
• W2048298097 cites W2136749613 @default.
• W2048298097 cites W2138954319 @default.
• W2048298097 cites W2160976967 @default.
• W2048298097 cites W2183821735 @default.
• W2048298097 cites W2224999886 @default.
• W2048298097 cites W2340804754 @default.
• W2048298097 cites W2942898131 @default.
• W2048298097 cites W32881753 @default.
• W2048298097 doi "https://doi.org/10.1016/s0011-2240(02)00034-2" @default.
• W2048298097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12237093" @default.
• W2048298097 hasPublicationYear "2002" @default.
• W2048298097 type Work @default.
• W2048298097 sameAs 2048298097 @default.
• W2048298097 citedByCount "25" @default.
• W2048298097 countsByYear W20482980972013 @default.
• W2048298097 countsByYear W20482980972014 @default.
• W2048298097 countsByYear W20482980972015 @default.
• W2048298097 countsByYear W20482980972016 @default.
• W2048298097 countsByYear W20482980972017 @default.
• W2048298097 countsByYear W20482980972018 @default.
• W2048298097 countsByYear W20482980972019 @default.
• W2048298097 countsByYear W20482980972020 @default.
• W2048298097 countsByYear W20482980972022 @default.
• W2048298097 crossrefType "journal-article" @default.
• W2048298097 hasAuthorship W2048298097A5023418784 @default.
• W2048298097 hasAuthorship W2048298097A5079889465 @default.
• W2048298097 hasAuthorship W2048298097A5090518514 @default.
• W2048298097 hasConcept C105702510 @default.
• W2048298097 hasConcept C131075544 @default.
• W2048298097 hasConcept C133605539 @default.
• W2048298097 hasConcept C142724271 @default.
• W2048298097 hasConcept C1491633281 @default.
• W2048298097 hasConcept C160160445 @default.
• W2048298097 hasConcept C16685009 @default.
• W2048298097 hasConcept C179933525 @default.
• W2048298097 hasConcept C181199279 @default.
• W2048298097 hasConcept C185592680 @default.
• W2048298097 hasConcept C196843134 @default.
• W2048298097 hasConcept C202751555 @default.
• W2048298097 hasConcept C2776350087 @default.
• W2048298097 hasConcept C2778260815 @default.
• W2048298097 hasConcept C2778394429 @default.
• W2048298097 hasConcept C2780070996 @default.
• W2048298097 hasConcept C53227056 @default.
• W2048298097 hasConcept C55493867 @default.
• W2048298097 hasConcept C66694092 @default.
• W2048298097 hasConcept C71924100 @default.
• W2048298097 hasConcept C74864618 @default.
• W2048298097 hasConcept C86803240 @default.
• W2048298097 hasConcept C95444343 @default.
• W2048298097 hasConceptScore W2048298097C105702510 @default.
• W2048298097 hasConceptScore W2048298097C131075544 @default.
• W2048298097 hasConceptScore W2048298097C133605539 @default.
• W2048298097 hasConceptScore W2048298097C142724271 @default.
• W2048298097 hasConceptScore W2048298097C1491633281 @default.
• W2048298097 hasConceptScore W2048298097C160160445 @default.
• W2048298097 hasConceptScore W2048298097C16685009 @default.
• W2048298097 hasConceptScore W2048298097C179933525 @default.
• W2048298097 hasConceptScore W2048298097C181199279 @default.
• W2048298097 hasConceptScore W2048298097C185592680 @default.
• W2048298097 hasConceptScore W2048298097C196843134 @default.
• W2048298097 hasConceptScore W2048298097C202751555 @default.
• W2048298097 hasConceptScore W2048298097C2776350087 @default.
• W2048298097 hasConceptScore W2048298097C2778260815 @default.
• W2048298097 hasConceptScore W2048298097C2778394429 @default.
• W2048298097 hasConceptScore W2048298097C2780070996 @default.
• W2048298097 hasConceptScore W2048298097C53227056 @default.
• W2048298097 hasConceptScore W2048298097C55493867 @default.
• W2048298097 hasConceptScore W2048298097C66694092 @default.
• W2048298097 hasConceptScore W2048298097C71924100 @default.
• W2048298097 hasConceptScore W2048298097C74864618 @default.
• W2048298097 hasConceptScore W2048298097C86803240 @default.
• W2048298097 hasConceptScore W2048298097C95444343 @default.
• W2048298097 hasIssue "3" @default.
• W2048298097 hasLocation W20482980971 @default.
• W2048298097 hasLocation W20482980972 @default.
• W2048298097 hasOpenAccess W2048298097 @default.
• W2048298097 hasPrimaryLocation W20482980971 @default.
• W2048298097 hasRelatedWork W1853467407 @default.
• W2048298097 hasRelatedWork W1971044127 @default.
• W2048298097 hasRelatedWork W2010635910 @default.

• next