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- W2048341736 abstract "In this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from Salmonella enteritidis in a yield of 3.9 mg/mL(-1) after being concentrated with polyethylene glycol (PEG). A monoclonal antibody (MAb), designated D7 clone, was generated from Balb/c mice immunized with Salmonella enteritidis flagella using the conventional hybridoma method. The D7 clone secreting MAb was classified as IgG2a isotype. ELISA analyses of D7 MAb to Salmonella-specific flagella demonstrated that the antibody reacted with H: m. However, Western immunoblot analyses of D7 clone appear to be secreting heavy chain of IgG2a antibody, which was eligible for the diagnosis of Salmonella enteritidis." @default.
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- W2048341736 date "2010-10-01" @default.
- W2048341736 modified "2023-10-18" @default.
- W2048341736 title "Production of Monoclonal Antibody Against Salmonella H: g,m Flagellar Antigen and Potential Diagnostic Application" @default.
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- W2048341736 doi "https://doi.org/10.1089/hyb.2010.0023" @default.
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