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• W2048348775 abstract "We have used dansyl chloride (5-dimethylamino-1-naphthalenesulfonyl choloride) to form dansyl derivatives of amine-containing compounds in follicular fluid or highly purified fractions containing a low molecular weight (MW) inhibitor of follicle-stimulating hormone (FSH) binding to receptor (FSH-BI). This approach allowed sensitive detection of the derivatives based on their fluorescent properties. By taking advantage of the hydrophobic nature of the dansyl group, a dansyl derivative (RF = 0.15) identified in low MW FSH-BI preparations was purified from porcine follicular fluid. Based on chromatographic criteria using four different systems (thin-layer chromatography [TLC] and high performance liquid chromatography), the derivatized factor (D15) that was purified appeared to be homogeneous. A direct, chemical assay was developed for quantification of D15 from follicular fluid or tissue extracts. The highest concentration (153 ng/mg) of D15 was found in ovarian tissue of adult rats, lesser amounts were observed in kidney and liver tissues (93 and 62 ng/mg, respectively) and even less in diaphram and heart tissues (5 and 0.5 ng/mg, respectively). High concentrations of D15 were observed in derivatized extracts of tests from immature rats in which approximately twice as much D15 was found in Leydig cells (241 ng/mg) as in seminiferous tubules (136 ng/mg). In porcine ovarian tissue, granulosa cells from large follicles and corpora lutea (69 and 91 ng/mg, respectively) contained at least 4-fold higher concentrations than follicle wall tissue (14 ng/ml). Relative concentrations of D15 material were also determined in pools of bovine follicular fluid previously shown to contain low MW FSH-BI. The concentration of D15 was highest (15.6 µg/ml) in fluid from small follicles obtained from cows during the luteal phase of the estrous cycle. These follicles also contained the highest concentration of low MW FSH-BI. No D15 was detected in fluid from large or small follicles from pregnant cows or from cystic follicles. These data indicate that low MW FSH-BI can be quantified by TLC after dansylation and suggest that high local concentrations exist in gonadal tissues, particularly luteinizing hormone-responsive cell types. On the basis of these data, the hypothesis that this factor is a local modulator (antagonist) of FSH action deserves further study." @default.
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• W2048348775 date "1989-02-01" @default.
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• W2048348775 title "Purification, Measurement, and Tissue Distribution of a Dansyl-Derivatized Glycopeptide from Low-Molecular Weight Follicle-Stimulating Hormone-Inhibitor-Containing Fractions of Porcine Follicular Fluid1" @default.
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• W2048348775 doi "https://doi.org/10.1095/biolreprod40.2.407" @default.
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