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• W2048350060 abstract "Abstract The Beckman Continuous Particle Electrophoresis instrument comprises a 0.15-cm thick, 5-cm wide, 50-cm long vertical channel through which electrolyte solution is pumped, entering at the top and exiting at the bottom through 48 horizontal tubes, 1 mm apart. The particle dispersion to be separated is injected as a cylindrical stream near the top of the channel and flows downward between two 30-cm long electrodes. When the voltage gradient is applied, the particle stream is displaced laterally according to the electrophoretic mobilities of the particles. If the distribution of electrophoretic mobilities is sufficiently broad, fractions of different electrophoretic mobility are collected. The fluid flow within the channel is controlled by the parabolic velocity profile in the verticle plane arising from the forced flow and the parabolic velocity profile in the horizontal plane arising from the electroosmotic flow. This instrument, designed for preparative electrophoresis, has been modified for analytical separations by an optical density scanner, which traverses the width of the channel. This analytical capability has been used to compare the experimental resolution of separation with that predicted by a mathematical model of the separation process. The experimental separations have been compared with theoretical predictions as a function of the experimental parameters investigated, especially the electroosmotic flow at the channel wall—liquid interface." @default.
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• W2048350060 date "1980-07-01" @default.
• W2048350060 modified "2023-09-27" @default.
• W2048350060 title "Experimental parameters involved in the separation of colloidal particles by continuous electrophoresis" @default.
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• W2048350060 doi "https://doi.org/10.1016/0166-6622(80)80024-2" @default.
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