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- W2048375056 abstract "PPi driven ATP synthesis has been reconstituted in a liposomal system containing the membrane-bound energy-linked PPiase and coupling factor complex, both highly purified from Rhodospirillum rubrum. This energy converting model system was made by mixing both enzyme preparations with an aqueous suspension of sonicated soybean phospholipids and subjecting to a freeze-thaw procedure. In the presence of ADP, Mg2+, Pi and PPi the system catalyzed phosphorylation by up to 25 nmol ATP formed X mg protein-1 X min-1, at 20 degrees C, which was sensitive to uncouplers and inhibitors of phosphorylation such as oligomycin, efrapeptin and N,N'-dicyclohexylcarbodiimide." @default.
- W2048375056 created "2016-06-24" @default.
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- W2048375056 date "1983-05-02" @default.
- W2048375056 modified "2023-10-03" @default.
- W2048375056 title "Inorganic pyrophosphate-driven ATP-synthesis in liposomes containing membrane-bound inorganic pyrophosphatase and F<sub>0</sub> -F<sub>1</sub> complex from <i>Rhodospirillum rubrum</i>" @default.
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- W2048375056 doi "https://doi.org/10.1016/0014-5793(83)80223-3" @default.
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