FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048389635> ?p ?o ?g. }

• W2048389635 endingPage "2940" @default.
• W2048389635 startingPage "2935" @default.
• W2048389635 abstract "Reports that interleukin-8 (IL-8) induces the infiltration of neutrophils followed by T-cells into injection sites led us to postulate that by stimulation of neutrophil degranulation IL-8 may cause the release of factors with chemoattractant activity for T-lymphocytes. Extracts of human neutrophil granules were chromatographed to isolate and purify T-lymphocyte chemoattractant factors. Two major peaks of T-cell chemotactic activity were purified by C18 reversed phase high pressure liquid chromatography (HPLC). The first peak was resolved further by C4 reversed phase HPLC and yielded an active fraction shown by NH2-terminal amino acid sequence analysis to contain defensins HNP-1, HNP-2, and HNP-3. Purified defensins HNP-1 and HNP-2 (kindly provided by Dr. R. I. Lehrer, UCLA) were also potent chemoattractants for human T-cells, while HNP-3 was inactive. The second peak of T-cell chemoattractant activity was also further purified to homogeneity by C4 reversed phase HPLC and identified by NH2-terminal sequence analysis as CAP37/azurocidin, a protein with sequence homology to serine proteases. 0.1-100 ng of defensins and 1.0-100 ng/ml CAP37 were able to stimulate in vitro T-cell chemotaxis. Neutrophil activating factors, i.e. IL-8, phorbol 12-myristate 13-acetate/ionomycin, and formylmethionylleucylphenylalanine each induced the release of CAP37 and defensins from neutrophil granules. Subcutaneous administration of defensins or CAP37/azurocidin into BALB/c mice resulted in a moderate neutrophil and mononuclear cell infiltrate by 4 h, which was greater by 24 h at the site of injection. Additionally, subcutaneous injection of defensins into chimeric huPBL-SCID mice resulted in significant infiltration by human CD3+ cells within 4 h. These results identify the antimicrobial proteins, CAP37/azurocidin and defensins HNP-1 and HNP-2, as potent neutrophil-derived chemoattractants for T-cells. These proteins represent primordial antimicrobial peptides which may have evolved into acute inflammatory cell-derived signals that mobilize immunocompetent T-cells and other inflammatory cells. Reports that interleukin-8 (IL-8) induces the infiltration of neutrophils followed by T-cells into injection sites led us to postulate that by stimulation of neutrophil degranulation IL-8 may cause the release of factors with chemoattractant activity for T-lymphocytes. Extracts of human neutrophil granules were chromatographed to isolate and purify T-lymphocyte chemoattractant factors. Two major peaks of T-cell chemotactic activity were purified by C18 reversed phase high pressure liquid chromatography (HPLC). The first peak was resolved further by C4 reversed phase HPLC and yielded an active fraction shown by NH2-terminal amino acid sequence analysis to contain defensins HNP-1, HNP-2, and HNP-3. Purified defensins HNP-1 and HNP-2 (kindly provided by Dr. R. I. Lehrer, UCLA) were also potent chemoattractants for human T-cells, while HNP-3 was inactive. The second peak of T-cell chemoattractant activity was also further purified to homogeneity by C4 reversed phase HPLC and identified by NH2-terminal sequence analysis as CAP37/azurocidin, a protein with sequence homology to serine proteases. 0.1-100 ng of defensins and 1.0-100 ng/ml CAP37 were able to stimulate in vitro T-cell chemotaxis. Neutrophil activating factors, i.e. IL-8, phorbol 12-myristate 13-acetate/ionomycin, and formylmethionylleucylphenylalanine each induced the release of CAP37 and defensins from neutrophil granules. Subcutaneous administration of defensins or CAP37/azurocidin into BALB/c mice resulted in a moderate neutrophil and mononuclear cell infiltrate by 4 h, which was greater by 24 h at the site of injection. Additionally, subcutaneous injection of defensins into chimeric huPBL-SCID mice resulted in significant infiltration by human CD3+ cells within 4 h. These results identify the antimicrobial proteins, CAP37/azurocidin and defensins HNP-1 and HNP-2, as potent neutrophil-derived chemoattractants for T-cells. These proteins represent primordial antimicrobial peptides which may have evolved into acute inflammatory cell-derived signals that mobilize immunocompetent T-cells and other inflammatory cells." @default.
• W2048389635 created "2016-06-24" @default.
• W2048389635 creator A5003750938 @default.
• W2048389635 creator A5035085941 @default.
• W2048389635 creator A5039707350 @default.
• W2048389635 creator A5042070203 @default.
• W2048389635 creator A5042240287 @default.
• W2048389635 creator A5060115409 @default.
• W2048389635 creator A5075003658 @default.
• W2048389635 creator A5076554157 @default.
• W2048389635 creator A5080826692 @default.
• W2048389635 date "1996-02-01" @default.
• W2048389635 modified "2023-10-08" @default.
• W2048389635 title "Identification of Defensin-1, Defensin-2, and CAP37/Azurocidin as T-cell Chemoattractant Proteins Released from Interleukin-8-stimulated Neutrophils" @default.
• W2048389635 cites W103686952 @default.
• W2048389635 cites W1524903400 @default.
• W2048389635 cites W1843968746 @default.
• W2048389635 cites W1893086372 @default.
• W2048389635 cites W1931276869 @default.
• W2048389635 cites W1975085160 @default.
• W2048389635 cites W1975742983 @default.
• W2048389635 cites W1982683980 @default.
• W2048389635 cites W1983239749 @default.
• W2048389635 cites W2002942416 @default.
• W2048389635 cites W2010599722 @default.
• W2048389635 cites W2027085000 @default.
• W2048389635 cites W2039137468 @default.
• W2048389635 cites W2055856930 @default.
• W2048389635 cites W2058902088 @default.
• W2048389635 cites W2072036820 @default.
• W2048389635 cites W2072771934 @default.
• W2048389635 cites W2080267955 @default.
• W2048389635 cites W2087069709 @default.
• W2048389635 cites W2089810387 @default.
• W2048389635 cites W2100295490 @default.
• W2048389635 cites W2111926316 @default.
• W2048389635 cites W2130950961 @default.
• W2048389635 cites W2142090875 @default.
• W2048389635 cites W2149880504 @default.
• W2048389635 cites W2158789628 @default.
• W2048389635 cites W2164722521 @default.
• W2048389635 cites W2176220887 @default.
• W2048389635 cites W4239357337 @default.
• W2048389635 cites W4296792904 @default.
• W2048389635 doi "https://doi.org/10.1074/jbc.271.6.2935" @default.
• W2048389635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8621683" @default.
• W2048389635 hasPublicationYear "1996" @default.
• W2048389635 type Work @default.
• W2048389635 sameAs 2048389635 @default.
• W2048389635 citedByCount "534" @default.
• W2048389635 countsByYear W20483896352012 @default.
• W2048389635 countsByYear W20483896352013 @default.
• W2048389635 countsByYear W20483896352014 @default.
• W2048389635 countsByYear W20483896352015 @default.
• W2048389635 countsByYear W20483896352016 @default.
• W2048389635 countsByYear W20483896352017 @default.
• W2048389635 countsByYear W20483896352018 @default.
• W2048389635 countsByYear W20483896352019 @default.
• W2048389635 countsByYear W20483896352020 @default.
• W2048389635 countsByYear W20483896352021 @default.
• W2048389635 countsByYear W20483896352022 @default.
• W2048389635 countsByYear W20483896352023 @default.
• W2048389635 crossrefType "journal-article" @default.
• W2048389635 hasAuthorship W2048389635A5003750938 @default.
• W2048389635 hasAuthorship W2048389635A5035085941 @default.
• W2048389635 hasAuthorship W2048389635A5039707350 @default.
• W2048389635 hasAuthorship W2048389635A5042070203 @default.
• W2048389635 hasAuthorship W2048389635A5042240287 @default.
• W2048389635 hasAuthorship W2048389635A5060115409 @default.
• W2048389635 hasAuthorship W2048389635A5075003658 @default.
• W2048389635 hasAuthorship W2048389635A5076554157 @default.
• W2048389635 hasAuthorship W2048389635A5080826692 @default.
• W2048389635 hasBestOaLocation W20483896351 @default.
• W2048389635 hasConcept C153911025 @default.
• W2048389635 hasConcept C170493617 @default.
• W2048389635 hasConcept C174106267 @default.
• W2048389635 hasConcept C185592680 @default.
• W2048389635 hasConcept C202751555 @default.
• W2048389635 hasConcept C203014093 @default.
• W2048389635 hasConcept C203565725 @default.
• W2048389635 hasConcept C2776498113 @default.
• W2048389635 hasConcept C2776914184 @default.
• W2048389635 hasConcept C2776989580 @default.
• W2048389635 hasConcept C2779281246 @default.
• W2048389635 hasConcept C49802076 @default.
• W2048389635 hasConcept C54166955 @default.
• W2048389635 hasConcept C55493867 @default.
• W2048389635 hasConcept C68324877 @default.
• W2048389635 hasConcept C86803240 @default.
• W2048389635 hasConceptScore W2048389635C153911025 @default.
• W2048389635 hasConceptScore W2048389635C170493617 @default.
• W2048389635 hasConceptScore W2048389635C174106267 @default.
• W2048389635 hasConceptScore W2048389635C185592680 @default.
• W2048389635 hasConceptScore W2048389635C202751555 @default.
• W2048389635 hasConceptScore W2048389635C203014093 @default.
• W2048389635 hasConceptScore W2048389635C203565725 @default.
• W2048389635 hasConceptScore W2048389635C2776498113 @default.
• W2048389635 hasConceptScore W2048389635C2776914184 @default.

• next