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• W2048510728 abstract "Based on knowledge from traditional Arab herbal medicine, this in vitro study aims to examine the anti-inflammatory mechanism of Hypericum triquetrifolium by measuring the expression and release of pro-inflammatory cytokines, tumor necrosis factor- α (TNF- α ) and interleukine-6 (IL-6), and inducible nitric oxide synthase (iNOS) in human monocytic cells, THP-1. The effects were assessed by measuring the levels of secretory proteins and mRNA of TNF- α and IL-6, the levels of nitric oxide (NO) secretion and the expression of iNOS in THP-1 cells. Cells were treated with 5 μ g lipopolysaccharide/ml (LPS) in the presence and absence of increasing concentrations of extracts from the aerial parts of H. triquetrifolium . During the entire experimental period, we used extract concentrations (up to 250 μ g mL −1 ) that had no cytotoxic effects, as measured with MTT and LDH assays. Hypericum triquetrifolium extracts remarkably suppressed the LPS-induced NO release, significantly attenuated the LPS-induced transcription of iNOS and inhibited in a dose-dependent manner the expression and release of TNF- α . No significant effects were observed on the release of IL-6. Taken together, these results suggest that H. triquetrifolium probably exerts anti-inflammatory effects through the suppression of TNF- α and iNOS expressions." @default.
• W2048510728 created "2016-06-24" @default.
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• W2048510728 date "2011-01-01" @default.
• W2048510728 modified "2023-09-24" @default.
• W2048510728 title "<i>Hypericum triquetrifolium</i>—Derived Factors Downregulate the Production Levels of LPS-Induced Nitric Oxide and Tumor Necrosis Factor-<i>α</i>in THP-1 Cells" @default.
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• W2048510728 doi "https://doi.org/10.1093/ecam/nen056" @default.
• W2048510728 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3136706" @default.
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