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• W2048574295 abstract "The P31T mutant of Staphylococcus aureus thioredoxin crystallizes spontaneously in space group P2(1)2(1)2(1), with unit-cell parameters a = 41.7, b = 49.5, c = 55.6 A. The crystals diffract to 2.2 A resolution. Isomorphous crystals of wild-type thioredoxin as well as of other point mutants only grow when seeded with the P31T mutant. These results suggest seeding as a valuable tool complementing surface engineering for proteins that are hard to crystallize." @default.
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• W2048574295 date "2006-11-30" @default.
• W2048574295 modified "2023-09-25" @default.
• W2048574295 title "Combining site-specific mutagenesis and seeding as a strategy to crystallize `difficult' proteins: the case of<i>Staphylococcus aureus</i>thioredoxin" @default.
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• W2048574295 doi "https://doi.org/10.1107/s1744309106047075" @default.
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