FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048576781> ?p ?o ?g. }

• W2048576781 endingPage "12290" @default.
• W2048576781 startingPage "12282" @default.
• W2048576781 abstract "The enzyme alpha-amino-beta-carboxy-muconic-epsilon-semialdehyde decarboxylase (ACMSD) plays an important role in the biodegradation of 2-nitrobenzoic acid in microorganisms and in tryptophan catabolism in humans. We report that the overexpressed ACMSD enzyme from Pseudomonas fluorescens requires a divalent metal, such as Co(II), Fe(II), Cd(II), or Mn(II), for catalytic activity and that neither a redox reagent nor an organic cofactor is required for the catalytic function. The metal ions can be taken up in either cell or cell-free preparations for generating the active form of ACMSD. The kinetic parameters and enzyme specific activity are shown to depend on the metal ion present in the enzyme, suggesting a catalytic role of the metal center. EPR spectrum of Co(II)-ACMSD provides a high-spin (S = 3/2 mononuclear metal ion in a non-heme, noncorrinoid environment with a mixed nitrogen/oxygen ligand field. We observe hyperfine interactions due to 59Co nucleus at temperatures below 5 K but not at higher temperatures. Ten hyperfine lines are present in the g(perpendicular) region, and three equivalent nitrogen hyperfine couplings are required to simulate the resonances in the EPR spectrum. The results for the metal binding site are also assessed using the copper-substituted enzyme, and the EPR spectral assignments for both cobalt and copper proteins give strong support for a distorted trigonal bipyramidal geometry of the metal center. Ultimately, these results suggest for the first time that ACMSD is a metal-dependent enzyme that catalyzes a novel nonoxidative decarboxylation." @default.
• W2048576781 created "2016-06-24" @default.
• W2048576781 creator A5007520893 @default.
• W2048576781 creator A5015693668 @default.
• W2048576781 creator A5036164858 @default.
• W2048576781 creator A5069795151 @default.
• W2048576781 creator A5071153074 @default.
• W2048576781 date "2005-08-10" @default.
• W2048576781 modified "2023-10-17" @default.
• W2048576781 title "Kinetic and Spectroscopic Characterization of ACMSD from <i>Pseudomonas fluorescens</i> Reveals a Pentacoordinate Mononuclear Metallocofactor" @default.
• W2048576781 cites W1481073719 @default.
• W2048576781 cites W1509679583 @default.
• W2048576781 cites W1572549182 @default.
• W2048576781 cites W1627869463 @default.
• W2048576781 cites W1890047923 @default.
• W2048576781 cites W1890299681 @default.
• W2048576781 cites W1915092483 @default.
• W2048576781 cites W1968419546 @default.
• W2048576781 cites W1969151014 @default.
• W2048576781 cites W1975144545 @default.
• W2048576781 cites W1978028485 @default.
• W2048576781 cites W1978939213 @default.
• W2048576781 cites W1982737489 @default.
• W2048576781 cites W1983985990 @default.
• W2048576781 cites W1986894327 @default.
• W2048576781 cites W1993565599 @default.
• W2048576781 cites W1994335443 @default.
• W2048576781 cites W2009885112 @default.
• W2048576781 cites W2012015421 @default.
• W2048576781 cites W2032278169 @default.
• W2048576781 cites W2051353156 @default.
• W2048576781 cites W2057192639 @default.
• W2048576781 cites W2062545296 @default.
• W2048576781 cites W2076689036 @default.
• W2048576781 cites W2087823869 @default.
• W2048576781 cites W2088004884 @default.
• W2048576781 cites W2092248171 @default.
• W2048576781 cites W2094313579 @default.
• W2048576781 cites W2095753590 @default.
• W2048576781 cites W2104499901 @default.
• W2048576781 cites W2128586767 @default.
• W2048576781 cites W2136124715 @default.
• W2048576781 cites W2161548658 @default.
• W2048576781 cites W2431495646 @default.
• W2048576781 cites W2950807855 @default.
• W2048576781 cites W3005439608 @default.
• W2048576781 cites W3192366276 @default.
• W2048576781 cites W4232957382 @default.
• W2048576781 doi "https://doi.org/10.1021/ja0532234" @default.
• W2048576781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16131206" @default.
• W2048576781 hasPublicationYear "2005" @default.
• W2048576781 type Work @default.
• W2048576781 sameAs 2048576781 @default.
• W2048576781 citedByCount "33" @default.
• W2048576781 countsByYear W20485767812012 @default.
• W2048576781 countsByYear W20485767812013 @default.
• W2048576781 countsByYear W20485767812014 @default.
• W2048576781 countsByYear W20485767812015 @default.
• W2048576781 countsByYear W20485767812016 @default.
• W2048576781 countsByYear W20485767812017 @default.
• W2048576781 countsByYear W20485767812018 @default.
• W2048576781 countsByYear W20485767812019 @default.
• W2048576781 countsByYear W20485767812020 @default.
• W2048576781 countsByYear W20485767812021 @default.
• W2048576781 countsByYear W20485767812023 @default.
• W2048576781 crossrefType "journal-article" @default.
• W2048576781 hasAuthorship W2048576781A5007520893 @default.
• W2048576781 hasAuthorship W2048576781A5015693668 @default.
• W2048576781 hasAuthorship W2048576781A5036164858 @default.
• W2048576781 hasAuthorship W2048576781A5069795151 @default.
• W2048576781 hasAuthorship W2048576781A5071153074 @default.
• W2048576781 hasConcept C116569031 @default.
• W2048576781 hasConcept C121332964 @default.
• W2048576781 hasConcept C170493617 @default.
• W2048576781 hasConcept C178790620 @default.
• W2048576781 hasConcept C179104552 @default.
• W2048576781 hasConcept C185592680 @default.
• W2048576781 hasConcept C187961010 @default.
• W2048576781 hasConcept C199164860 @default.
• W2048576781 hasConcept C46141821 @default.
• W2048576781 hasConcept C544153396 @default.
• W2048576781 hasConcept C55493867 @default.
• W2048576781 hasConcept C71240020 @default.
• W2048576781 hasConcept C75473681 @default.
• W2048576781 hasConceptScore W2048576781C116569031 @default.
• W2048576781 hasConceptScore W2048576781C121332964 @default.
• W2048576781 hasConceptScore W2048576781C170493617 @default.
• W2048576781 hasConceptScore W2048576781C178790620 @default.
• W2048576781 hasConceptScore W2048576781C179104552 @default.
• W2048576781 hasConceptScore W2048576781C185592680 @default.
• W2048576781 hasConceptScore W2048576781C187961010 @default.
• W2048576781 hasConceptScore W2048576781C199164860 @default.
• W2048576781 hasConceptScore W2048576781C46141821 @default.
• W2048576781 hasConceptScore W2048576781C544153396 @default.
• W2048576781 hasConceptScore W2048576781C55493867 @default.
• W2048576781 hasConceptScore W2048576781C71240020 @default.
• W2048576781 hasConceptScore W2048576781C75473681 @default.
• W2048576781 hasIssue "35" @default.

• next