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- W2048581637 abstract "Summary The 17 kb kps gene cluster of Escherichia coli K1, which encodes the information required for synthesis, assembly and translocation of the polysialic acid capsule of E. coli K1, is divided into three functional regions. Region 3 contains two genes, kpsM and kpsT , essential for the transport of capsule polymer across the cytoplasmic membrane. The hydrophobicity profile of KpsM suggests that it is an integral membrane protein while KpsT contains a consensus ATP‐binding site. KpsM and KpsT belong to the ATP‐binding cassette (ABC) superfamily of membrane transporters. In this study, we investigate the topology of KpsM within the cytoplasmic membrane using β‐lactamase fusions and alkaline phosphatase sandwich fusions. Our analysis provides evidence for a model of KpsM having six membrane‐spanning regions, with the N ‐ and C ‐terminal domains facing the cytoplasm, and a short domain within the third periplasmic loop, which we refer to as the SV–SVI linker localizing in the membrane. Protease digestion studies are consistent with regions of KpsM exposed to the periplasmic space. In vivo cross‐linking studies provide support for dimerization of KpsM within the cytoplasmic membrane. Linker‐insertion and site‐directed mutagenesis define the N ‐terminus, the first cytoplasmic loop, and the SV‐SVI linker as regions that are important for the function of KpsM in K1 polymer transport." @default.
- W2048581637 created "2016-06-24" @default.
- W2048581637 creator A5031109212 @default.
- W2048581637 creator A5036955854 @default.
- W2048581637 date "1994-12-01" @default.
- W2048581637 modified "2023-09-29" @default.
- W2048581637 title "Topological and mutational analysis of KpsM, the hydrophobic component of the ABC-transporter involved in the export of polysialic acid in Escherichia coli K1" @default.
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- W2048581637 doi "https://doi.org/10.1111/j.1365-2958.1994.tb01323.x" @default.
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