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• W2048602020 abstract "We investigated the effects of TNF receptor 1 (TNFR1) and 2 (TNFR2) modulation on the death of human aortic endothelial cells (HAECs) resistant to TNF-alpha-induced cell death. Alteration of the transcription of anti-apoptotic proteins, including inhibitor of apoptosis protein 1, 2 (cIAP1, 2), TNF receptor-associated factor 1 (TRAF1), nuclear factor kappa B1 protein (NFkappaB1), and FLICE-inhibitory protein (FLIP) was assessed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). TNF-alpha (200 ng/ml) or actinomycin D (ActD) (5 ng/ml) did not kill cells, while 5 ng/ml of TNF-alpha and 5 ng/ml of ActD increased expression of Fas (CD95) and FasL (CD95L), and 45% of cells died. TNFR2 blockade suppressed NFkappaB1 and FLIP expression and increased cell death. TNFR1 blockade enhanced FLIP expression and decreased cell death. Cells insensitive to TNF-alpha may respond to TNF-alpha through TNFR that induces transcription of NFkappaB1 and FLIP. Down-regulation of these proteins may facilitate death of cells insensitive to TNF-alpha-induced cell death." @default.
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• W2048602020 date "2001-07-01" @default.
• W2048602020 modified "2023-09-22" @default.
• W2048602020 title "REDUCED EXPRESSION OF FLICE-INHIBITORY PROTEIN (FLIP) AND NFκB IS ASSOCIATED WITH DEATH RECEPTOR-INDUCED CELL DEATH IN HUMAN AORTIC ENDOTHELIAL CELLS (HAECs)" @default.
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• W2048602020 doi "https://doi.org/10.1006/cyto.2001.0916" @default.
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