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• W2048606972 abstract "Matrix metalloproteinase-2 (MMP-2) plays a role in agonist- and tumour cell-induced platelet aggregation (TCIPA). MMP-2 is synthesized as a proenzyme and is activated at the cell surface by membrane type-1 matrix metalloproteinase (MT1-MMP, MMP-14). The significance of tumour cell-associated MT1-MMP for TCIPA was investigated using human breast carcinoma MCF7 cells stably coexpressing the integrin αvβ3 with MT1-MMP, cells expressing αvβ3 alone and mock-transfected cells. Western blot and zymography confirmed that αvβ3/MT1-MMP cells expressed MT1-MMP and efficiently processed proMMP-2 to MMP-2. Aggregometry, phase-contrast and transmission electron microscopy and flow cytometry were used to characterize TCIPA induced by MCF7 cell lines. The aggregating potency of cells was: αvβ3/MT1-MMP >αvβ3=mock cells, as shown by aggregometry and phase-contrast microscopy. Electron microscopy revealed close, membrane–membrane interactions between activated platelets and αvβ3/MT1-MMP cells during TCIPA. Inhibition of MMP-2 with the neutralizing anti-MMP-2 antibody (5 μg ml−1) and o-phenanthroline (100 μM) reduced aggregation induced by αvβ3/MT1-MMP cells. TCIPA induced by αvβ3/MT1-MMP cells was also reduced by inhibiting the generation and actions of ADP with apyrase (250 μg ml−1) and 2-methylthio-AMP (2-MeSAMP) (30 μM), but not N6-methyl-2′-deoxyadenosine-3′,5′-bisphosphate (MRS2179) (30 μM). Flow cytometry demonstrated that TCIPA enhanced expression of glycoprotein (GP) Ib and IIb/IIIa receptors not only on platelets but also on breast cancer cells. Thus, (a) human breast carcinoma cell surface-associated MT1-MMP, via activating proMMP-2, stimulates TCIPA; (b) ADP amplifies the effects of MMPs via stimulation of P2Y12 receptors and (c) both tumour- and platelet-derived GPIb and GPIIb/IIIa are involved in the aggregatory effects of MT1-MMP. British Journal of Pharmacology (2004) 141, 241–252. doi:10.1038/sj.bjp.0705606" @default.
• W2048606972 created "2016-06-24" @default.
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• W2048606972 date "2004-01-01" @default.
• W2048606972 modified "2023-10-16" @default.
• W2048606972 title "Membrane type-1 matrix metalloproteinase stimulates tumour cell-induced platelet aggregation: role of receptor glycoproteins" @default.
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• W2048606972 cites W1550232108 @default.
• W2048606972 cites W1585714318 @default.
• W2048606972 cites W1600560134 @default.
• W2048606972 cites W1684240761 @default.
• W2048606972 cites W1757310287 @default.
• W2048606972 cites W1786914907 @default.
• W2048606972 cites W1880636970 @default.
• W2048606972 cites W1895802579 @default.
• W2048606972 cites W1905017519 @default.
• W2048606972 cites W1931073059 @default.
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• W2048606972 cites W1970314984 @default.
• W2048606972 cites W1979390430 @default.
• W2048606972 cites W1987672928 @default.
• W2048606972 cites W1995954397 @default.
• W2048606972 cites W2001165687 @default.
• W2048606972 cites W2003013848 @default.
• W2048606972 cites W2005947955 @default.
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• W2048606972 cites W2008834972 @default.
• W2048606972 cites W2008971450 @default.
• W2048606972 cites W2009400023 @default.
• W2048606972 cites W2027473346 @default.
• W2048606972 cites W2029231027 @default.
• W2048606972 cites W2033334122 @default.
• W2048606972 cites W2035088036 @default.
• W2048606972 cites W2036145275 @default.
• W2048606972 cites W2040166170 @default.
• W2048606972 cites W2049220062 @default.
• W2048606972 cites W2056447026 @default.
• W2048606972 cites W2056698088 @default.
• W2048606972 cites W2058906406 @default.
• W2048606972 cites W2059910197 @default.
• W2048606972 cites W2062878537 @default.
• W2048606972 cites W2068561314 @default.
• W2048606972 cites W2070099006 @default.
• W2048606972 cites W2070312129 @default.
• W2048606972 cites W2072995607 @default.
• W2048606972 cites W2073102229 @default.
• W2048606972 cites W2079323372 @default.
• W2048606972 cites W2089550566 @default.
• W2048606972 cites W2090406032 @default.
• W2048606972 cites W2092378032 @default.
• W2048606972 cites W2100607142 @default.
• W2048606972 cites W2104063371 @default.
• W2048606972 cites W2114396237 @default.
• W2048606972 cites W2118523009 @default.
• W2048606972 cites W2121513486 @default.
• W2048606972 cites W2128049766 @default.
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• W2048606972 cites W2304874055 @default.
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• W2048606972 cites W2407760336 @default.
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• W2048606972 doi "https://doi.org/10.1038/sj.bjp.0705606" @default.
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