FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048610030> ?p ?o ?g. }

• W2048610030 endingPage "572" @default.
• W2048610030 startingPage "564" @default.
• W2048610030 abstract "Abstract Alteromonas haloplanctis is a bacterium that flourishes in Antarctic sea‐water and it is considered as an extreme psychrophile. We have determined the crystal structures of the α‐amylase (AHA) secreted by this bacterium, in its native state to 2.0 Å resolution as well as in complex with Tris to 1.85 Å resolution. The structure of AHA, which is the first experimentally determined three‐dimensional structure of a psychrophilic enzyme, resembles those of other known α‐amylases of various origins with a surprisingly greatest similarity to mammalian α‐amylases. AHA contains a chlorideion which activates the hydrolytic cleavage of substrate α‐l,4‐glycosidic bonds. The chloride binding site is situated ∼5 Å from the active site which is characterized by a triad of acid residues (Asp 174, Glu 200, Asp 264). These are all involved in firm binding of the Tris moiety. A reaction mechanism for substrate hydrolysis is proposed on the basis of the Tris inhibitor binding and the chloride activation.A trio of residues (Ser 303, His 337, Glu 19) having a striking spatial resemblance with serine‐protease like catalytic triads was found ∼22 Å from the active site. We found that this triad is equally present in other chloride dependent α‐amylases, and suggest that it could be responsible for autoproteolytic events observed in solution for this cold adapted α‐amylase." @default.
• W2048610030 created "2016-06-24" @default.
• W2048610030 creator A5008336818 @default.
• W2048610030 creator A5020954193 @default.
• W2048610030 creator A5059310855 @default.
• W2048610030 creator A5077001458 @default.
• W2048610030 date "1998-03-01" @default.
• W2048610030 modified "2023-10-15" @default.
• W2048610030 title "Crystal structures of the psychrophilic α‐amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor" @default.
• W2048610030 cites W1481344232 @default.
• W2048610030 cites W1523758704 @default.
• W2048610030 cites W1591425165 @default.
• W2048610030 cites W1597150658 @default.
• W2048610030 cites W1918349277 @default.
• W2048610030 cites W1925779720 @default.
• W2048610030 cites W1969600218 @default.
• W2048610030 cites W1975914851 @default.
• W2048610030 cites W1979151694 @default.
• W2048610030 cites W1987250042 @default.
• W2048610030 cites W1987308950 @default.
• W2048610030 cites W1989455203 @default.
• W2048610030 cites W1991781000 @default.
• W2048610030 cites W1993217483 @default.
• W2048610030 cites W1996607909 @default.
• W2048610030 cites W1998869143 @default.
• W2048610030 cites W2005080525 @default.
• W2048610030 cites W2015055160 @default.
• W2048610030 cites W2019429599 @default.
• W2048610030 cites W2033809613 @default.
• W2048610030 cites W2035650331 @default.
• W2048610030 cites W2039339358 @default.
• W2048610030 cites W2039664027 @default.
• W2048610030 cites W2046319395 @default.
• W2048610030 cites W2052344553 @default.
• W2048610030 cites W2060262553 @default.
• W2048610030 cites W2060448324 @default.
• W2048610030 cites W2064142837 @default.
• W2048610030 cites W2070598555 @default.
• W2048610030 cites W2080476827 @default.
• W2048610030 cites W2081035326 @default.
• W2048610030 cites W2086442729 @default.
• W2048610030 cites W2091075510 @default.
• W2048610030 cites W2093243013 @default.
• W2048610030 cites W2101749711 @default.
• W2048610030 cites W2105211035 @default.
• W2048610030 cites W2113473027 @default.
• W2048610030 cites W2116275717 @default.
• W2048610030 cites W2118063339 @default.
• W2048610030 cites W2127670645 @default.
• W2048610030 cites W2135225501 @default.
• W2048610030 cites W2151822784 @default.
• W2048610030 cites W2415586475 @default.
• W2048610030 cites W4300919461 @default.
• W2048610030 doi "https://doi.org/10.1002/pro.5560070304" @default.
• W2048610030 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2143949" @default.
• W2048610030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9541387" @default.
• W2048610030 hasPublicationYear "1998" @default.
• W2048610030 type Work @default.
• W2048610030 sameAs 2048610030 @default.
• W2048610030 citedByCount "176" @default.
• W2048610030 countsByYear W20486100302012 @default.
• W2048610030 countsByYear W20486100302013 @default.
• W2048610030 countsByYear W20486100302014 @default.
• W2048610030 countsByYear W20486100302015 @default.
• W2048610030 countsByYear W20486100302016 @default.
• W2048610030 countsByYear W20486100302017 @default.
• W2048610030 countsByYear W20486100302019 @default.
• W2048610030 countsByYear W20486100302020 @default.
• W2048610030 countsByYear W20486100302021 @default.
• W2048610030 countsByYear W20486100302022 @default.
• W2048610030 countsByYear W20486100302023 @default.
• W2048610030 crossrefType "journal-article" @default.
• W2048610030 hasAuthorship W2048610030A5008336818 @default.
• W2048610030 hasAuthorship W2048610030A5020954193 @default.
• W2048610030 hasAuthorship W2048610030A5059310855 @default.
• W2048610030 hasAuthorship W2048610030A5077001458 @default.
• W2048610030 hasBestOaLocation W20486100302 @default.
• W2048610030 hasConcept C107824862 @default.
• W2048610030 hasConcept C145770059 @default.
• W2048610030 hasConcept C150487720 @default.
• W2048610030 hasConcept C170835558 @default.
• W2048610030 hasConcept C181199279 @default.
• W2048610030 hasConcept C185592680 @default.
• W2048610030 hasConcept C22802001 @default.
• W2048610030 hasConcept C2776568683 @default.
• W2048610030 hasConcept C2778926719 @default.
• W2048610030 hasConcept C2780120296 @default.
• W2048610030 hasConcept C41183919 @default.
• W2048610030 hasConcept C523546767 @default.
• W2048610030 hasConcept C54355233 @default.
• W2048610030 hasConcept C55493867 @default.
• W2048610030 hasConcept C71240020 @default.
• W2048610030 hasConcept C86803240 @default.
• W2048610030 hasConceptScore W2048610030C107824862 @default.
• W2048610030 hasConceptScore W2048610030C145770059 @default.
• W2048610030 hasConceptScore W2048610030C150487720 @default.
• W2048610030 hasConceptScore W2048610030C170835558 @default.
• W2048610030 hasConceptScore W2048610030C181199279 @default.

• next