Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048625796> ?p ?o ?g. }
- W2048625796 endingPage "211" @default.
- W2048625796 startingPage "191" @default.
- W2048625796 abstract "Bacterial endotoxins such as lipopolysaccharides (LPS) are major contaminants of most pharmaceutical and consumer products. We report an antibiotic-mediated plasmonic biosensor for LPS detection based on a facile U-bent fiber optic probe (UFOP) technology. Biomimetic self-assembled layer of octadecyltrichlorosilanes (OTS) were functionalized on the surface of optical fiber probes to hydrophobically entrap LPS from aqueous solutions. The binding of LPS molecules was monitored in real-time by measuring the change in refractive index (RI) in the evanescent layer. To add specificity and signal amplification, the bound LPS molecules were further tagged with antimicrobial polymyxin-B conjugated gold nanoparticles (PMB-AuNPs) in a sandwich format. The assay was extensively optimized by investigating the role of experimental parameters like OTS concentration, incubation time and addition of a silver reduction step at the end of the assay. The lower limit of detection (LOD) for LPS was found to be 0.4 ng/mL with a 36-fold improved sensitivity upon silver enhancement. The total assay time was 1 h. The assay was also found to be highly specific in the presence of common biopharmaceutical components and could thus serve as an efficient endotoxin detection platform for quality control testing during therapeutic development." @default.
- W2048625796 created "2016-06-24" @default.
- W2048625796 creator A5005419394 @default.
- W2048625796 creator A5022814723 @default.
- W2048625796 creator A5060797748 @default.
- W2048625796 creator A5083984449 @default.
- W2048625796 date "1993-04-01" @default.
- W2048625796 modified "2023-10-03" @default.
- W2048625796 title "Phase behavior, supramolecular structure, and molecular conformation of lipopolysaccharide" @default.
- W2048625796 cites W1488656886 @default.
- W2048625796 cites W1582984670 @default.
- W2048625796 cites W1590916512 @default.
- W2048625796 cites W1606581707 @default.
- W2048625796 cites W1733288684 @default.
- W2048625796 cites W1855798100 @default.
- W2048625796 cites W1865883256 @default.
- W2048625796 cites W1923128572 @default.
- W2048625796 cites W1923688626 @default.
- W2048625796 cites W1967379808 @default.
- W2048625796 cites W1980970487 @default.
- W2048625796 cites W1982483138 @default.
- W2048625796 cites W1983130027 @default.
- W2048625796 cites W1994994154 @default.
- W2048625796 cites W1996653238 @default.
- W2048625796 cites W1998357541 @default.
- W2048625796 cites W1999698653 @default.
- W2048625796 cites W2001766506 @default.
- W2048625796 cites W2020415715 @default.
- W2048625796 cites W2024488580 @default.
- W2048625796 cites W2025441771 @default.
- W2048625796 cites W2029157767 @default.
- W2048625796 cites W2033322066 @default.
- W2048625796 cites W2035207969 @default.
- W2048625796 cites W2036624477 @default.
- W2048625796 cites W2040684628 @default.
- W2048625796 cites W2044942710 @default.
- W2048625796 cites W2051763511 @default.
- W2048625796 cites W2063978906 @default.
- W2048625796 cites W2065934807 @default.
- W2048625796 cites W2068865225 @default.
- W2048625796 cites W2076429952 @default.
- W2048625796 cites W2081566990 @default.
- W2048625796 cites W2084858354 @default.
- W2048625796 cites W2088633393 @default.
- W2048625796 cites W2093694682 @default.
- W2048625796 cites W2099119150 @default.
- W2048625796 cites W2116771540 @default.
- W2048625796 cites W2134377628 @default.
- W2048625796 cites W2145168099 @default.
- W2048625796 cites W2154744978 @default.
- W2048625796 cites W2165852855 @default.
- W2048625796 cites W2332123057 @default.
- W2048625796 cites W2408246646 @default.
- W2048625796 cites W2411146646 @default.
- W2048625796 doi "https://doi.org/10.1016/s0171-2985(11)80339-6" @default.
- W2048625796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8330897" @default.
- W2048625796 hasPublicationYear "1993" @default.
- W2048625796 type Work @default.
- W2048625796 sameAs 2048625796 @default.
- W2048625796 citedByCount "103" @default.
- W2048625796 countsByYear W20486257962013 @default.
- W2048625796 countsByYear W20486257962014 @default.
- W2048625796 countsByYear W20486257962017 @default.
- W2048625796 countsByYear W20486257962018 @default.
- W2048625796 countsByYear W20486257962019 @default.
- W2048625796 crossrefType "journal-article" @default.
- W2048625796 hasAuthorship W2048625796A5005419394 @default.
- W2048625796 hasAuthorship W2048625796A5022814723 @default.
- W2048625796 hasAuthorship W2048625796A5060797748 @default.
- W2048625796 hasAuthorship W2048625796A5083984449 @default.
- W2048625796 hasConcept C104819515 @default.
- W2048625796 hasConcept C119128265 @default.
- W2048625796 hasConcept C12554922 @default.
- W2048625796 hasConcept C128972844 @default.
- W2048625796 hasConcept C134018914 @default.
- W2048625796 hasConcept C153911025 @default.
- W2048625796 hasConcept C155672457 @default.
- W2048625796 hasConcept C160756335 @default.
- W2048625796 hasConcept C171250308 @default.
- W2048625796 hasConcept C185592680 @default.
- W2048625796 hasConcept C192562407 @default.
- W2048625796 hasConcept C21951064 @default.
- W2048625796 hasConcept C2778754761 @default.
- W2048625796 hasConcept C2780026444 @default.
- W2048625796 hasConcept C2780675486 @default.
- W2048625796 hasConcept C43617362 @default.
- W2048625796 hasConcept C501593827 @default.
- W2048625796 hasConcept C55493867 @default.
- W2048625796 hasConcept C71924100 @default.
- W2048625796 hasConcept C86803240 @default.
- W2048625796 hasConceptScore W2048625796C104819515 @default.
- W2048625796 hasConceptScore W2048625796C119128265 @default.
- W2048625796 hasConceptScore W2048625796C12554922 @default.
- W2048625796 hasConceptScore W2048625796C128972844 @default.
- W2048625796 hasConceptScore W2048625796C134018914 @default.
- W2048625796 hasConceptScore W2048625796C153911025 @default.
- W2048625796 hasConceptScore W2048625796C155672457 @default.
- W2048625796 hasConceptScore W2048625796C160756335 @default.