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- W2048658625 abstract "Plant regeneration has been achieved by somatic ernbryogenesis in Medicago trautwettery (Summ.). High number of somatic embryos were obtained from cultured cotyledones of M. trautwettery var. T1 seedlingls on modified B5 medium (Gamborg et al., 1968) containing 5 mg/1 2,4-D and 0.5 mg/1 kinetin (B5E), either within callus initiation (agar culture) or directly from the explant tissue in liquid B5E medium (Drop culture). Highly embryogenic cell suspension was achieved by cultivation of callus in liquid B5E medium. The embryos at globular-shaped stage were cultivated for further development in B53M/PEG (Denchev et al., 1990). Embryo maturation was carried out for two weeks on 0.6% agar MS (Murashige and Skoog, 1962) medium containing 30 μM ABA. Somatic embryo conversion was achieved by transferring the embryos onto MS medium supplemented with 27 μM GA3. Plant regeneration was carried out on 0.9% agar MS basal medium with 10 g/1 sucrose." @default.
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- W2048658625 date "1991-01-01" @default.
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- W2048658625 title "A Tissue Cultures System for High Frequency Somatic Embryogenesis in Medicago Trautwettery" @default.
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- W2048658625 doi "https://doi.org/10.1080/13102818.1991.10818632" @default.
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