FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048708098> ?p ?o ?g. }

• W2048708098 abstract "Actin filaments (F-actin) interact with myosin and activate its ATPase to support force generation. By comparing crystal structures of G-actin and the quasi-atomic model of F-actin based on high-resolution cryo-electron microscopy, the tyrosine-143 was found to be exposed more than 60Å(2) to the solvent in F-actin. Because tyrosine-143 flanks the hydrophobic cleft near the hydrophobic helix that binds to myosin, the mutant actins, of which the tyrosine-143 was replaced with tryptophan, phenylalanine, or isoleucine, were generated using the Dictyostelium expression system. It polymerized significantly poorly when induced by NaCl, but almost normally by KCl. In the presence of phalloidin and KCl, the extents of the polymerization of all the mutant actins were comparable to that of the wild-type actin so that the actin-activated myosin ATPase activity could be reliably compared. The affinity of skeletal heavy meromyosin to F-actin and the maximum ATPase activity (Vmax) were estimated by a double reciprocal plot. The Tyr143Trp-actin showed the higher affinity (smaller Kapp) than that of the wild-type actin, with the Vmax being almost unchanged. The Kapp and Vmax of the Tyr143Phe-actin were similar to those of the wild-type actin. However, the activation by Tyr143Ile-actin was much smaller than the wild-type actin and the accurate determination of Kapp was difficult. Comparison of the myosin ATPase activated by the various mutant actins at the same concentration of F-actin showed that the extent of activation correlates well with the solvent-accessible surface areas (ASA) of the replaced amino acid molecule. Because 1/Kapp reflects the affinity of F-actin for the myosin-ADP-phosphate intermediate (M.ADP.Pi) through the weak binding, these data suggest that the bulkiness or the aromatic nature of the tyrosin-143 is important for the initial binding of the M.ADP.Pi intermediate with F-actin but not for later processes such as the phosphate release." @default.
• W2048708098 created "2016-06-24" @default.
• W2048708098 creator A5025588595 @default.
• W2048708098 creator A5072362386 @default.
• W2048708098 creator A5074349994 @default.
• W2048708098 date "2013-11-01" @default.
• W2048708098 modified "2023-09-24" @default.
• W2048708098 title "Bulkiness or aromatic nature of tyrosine-143 of actin is important for the weak binding between F-actin and myosin-ADP-phosphate" @default.
• W2048708098 cites W1757420305 @default.
• W2048708098 cites W1890794327 @default.
• W2048708098 cites W1966349701 @default.
• W2048708098 cites W1972106355 @default.
• W2048708098 cites W1993524050 @default.
• W2048708098 cites W2006168221 @default.
• W2048708098 cites W2006494363 @default.
• W2048708098 cites W2024588129 @default.
• W2048708098 cites W2025210292 @default.
• W2048708098 cites W2032958472 @default.
• W2048708098 cites W2044629144 @default.
• W2048708098 cites W2051564364 @default.
• W2048708098 cites W2056478465 @default.
• W2048708098 cites W2068265376 @default.
• W2048708098 cites W2099540110 @default.
• W2048708098 cites W2103911415 @default.
• W2048708098 cites W2112590743 @default.
• W2048708098 cites W2125682735 @default.
• W2048708098 cites W2132629607 @default.
• W2048708098 cites W2139229287 @default.
• W2048708098 cites W2140040046 @default.
• W2048708098 cites W2291087670 @default.
• W2048708098 doi "https://doi.org/10.1016/j.bbrc.2013.10.144" @default.
• W2048708098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24211213" @default.
• W2048708098 hasPublicationYear "2013" @default.
• W2048708098 type Work @default.
• W2048708098 sameAs 2048708098 @default.
• W2048708098 citedByCount "0" @default.
• W2048708098 crossrefType "journal-article" @default.
• W2048708098 hasAuthorship W2048708098A5025588595 @default.
• W2048708098 hasAuthorship W2048708098A5072362386 @default.
• W2048708098 hasAuthorship W2048708098A5074349994 @default.
• W2048708098 hasConcept C102091540 @default.
• W2048708098 hasConcept C12554922 @default.
• W2048708098 hasConcept C125705527 @default.
• W2048708098 hasConcept C15954220 @default.
• W2048708098 hasConcept C181199279 @default.
• W2048708098 hasConcept C185592680 @default.
• W2048708098 hasConcept C23265538 @default.
• W2048708098 hasConcept C2776165026 @default.
• W2048708098 hasConcept C55493867 @default.
• W2048708098 hasConcept C6997183 @default.
• W2048708098 hasConcept C8035138 @default.
• W2048708098 hasConcept C86803240 @default.
• W2048708098 hasConceptScore W2048708098C102091540 @default.
• W2048708098 hasConceptScore W2048708098C12554922 @default.
• W2048708098 hasConceptScore W2048708098C125705527 @default.
• W2048708098 hasConceptScore W2048708098C15954220 @default.
• W2048708098 hasConceptScore W2048708098C181199279 @default.
• W2048708098 hasConceptScore W2048708098C185592680 @default.
• W2048708098 hasConceptScore W2048708098C23265538 @default.
• W2048708098 hasConceptScore W2048708098C2776165026 @default.
• W2048708098 hasConceptScore W2048708098C55493867 @default.
• W2048708098 hasConceptScore W2048708098C6997183 @default.
• W2048708098 hasConceptScore W2048708098C8035138 @default.
• W2048708098 hasConceptScore W2048708098C86803240 @default.
• W2048708098 hasFunder F4320320912 @default.
• W2048708098 hasLocation W20487080981 @default.
• W2048708098 hasLocation W20487080982 @default.
• W2048708098 hasOpenAccess W2048708098 @default.
• W2048708098 hasPrimaryLocation W20487080981 @default.
• W2048708098 hasRelatedWork W1601407224 @default.
• W2048708098 hasRelatedWork W1753223793 @default.
• W2048708098 hasRelatedWork W1972823922 @default.
• W2048708098 hasRelatedWork W1987486381 @default.
• W2048708098 hasRelatedWork W1994466407 @default.
• W2048708098 hasRelatedWork W20108084 @default.
• W2048708098 hasRelatedWork W2016825470 @default.
• W2048708098 hasRelatedWork W2044403344 @default.
• W2048708098 hasRelatedWork W2736266827 @default.
• W2048708098 hasRelatedWork W2480246289 @default.
• W2048708098 isParatext "false" @default.
• W2048708098 isRetracted "false" @default.
• W2048708098 magId "2048708098" @default.
• W2048708098 workType "article" @default.