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• W2048721368 abstract "Conditions for high-cell-density fermentations of Saccharomyces cerevisiae strains producing recombinant-DNA-derived proteins were established. Strains producing human immune interferon (IFN-γ) from the constitutive PGK promoter failed to grow to high cell densities and exhibited low plasmid stability. Regulated expression of IFN-γ was obtained in similar strains by employing a hybrid yeast GPD promoter that was subject to carbon source regulation due to the presence of regulatory DNA sequences from the yeast GAL 1,10 intergenic region. IFN-γ expression programmed by this vector was low during growth on glucose and was induced by galactose. Previously defined fermentation conditions employing glucose as a carbon source were applied to this strain, resulting in high ceil densities with higher plasmid stability. Various methods of galactose induction of IFN-γ expression in high-cell-density fermentations were investigated. Optimal conditions resulted in a 2000-fold induction and production of 2 g IFN-γ/L fermentation culture." @default.
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• W2048721368 date "1987-06-20" @default.
• W2048721368 modified "2023-10-10" @default.
• W2048721368 title "Controlled expression and purification of human immune interferon from high-cell-density fermentations ofSaccharomyces cerevisiae" @default.
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• W2048721368 doi "https://doi.org/10.1002/bit.260290911" @default.
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