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• W2048755369 abstract "In this Letter, we demonstrate that chemical oxidation polymerization of o-phenylenediamine (OPD) by potassium bichromate at room temperature results in the formation of submicrometer-scale poly(o-phenylenediamine) (POPD) colloids. Such colloids can absorb and quench dye-labeled single-stranded DNA (ssDNA) very effectively. In the presence of a target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the POPD surface, leading to recovery of dye fluorescence. With the use of an oligonucleotide (OND) sequence associated with human immunodeficiency virus (HIV) as a model system, we demonstrate the proof of concept that POPD colloid-quenched fluorescent OND can be used as a probe for fluorescence-enhanced nucleic acid detection with selectivity down to single-base mismatch." @default.
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• W2048755369 date "2010-12-27" @default.
• W2048755369 modified "2023-10-18" @default.
• W2048755369 title "Poly(<i>o</i>-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection" @default.
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• W2048755369 doi "https://doi.org/10.1021/la103799e" @default.
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