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- W2048770748 abstract "Abstract This paper describes the synthesis and study of a new fluorigenic, thiol-specific reagent, ammonium 4-chloro-7-sulfobenzofurazan, which is readily prepared by sulfonation of 4-chlorobenzofurazan. Evaluation of ammonium 4-chloro-7-sulfobenzofurazan was undertaken using glutathione as a model thiol peptide and bovine serum albumin and jackbean urease as thiol-containing proteins. Thiol specificity of this reagent was established using various amino acids and peptides including l -cysteine, l -lysine, l -histidine, l -tyrosine, glutathione, and oxidized glutathione. Nitrogen and sulfur derivatives of ethanediol and acetic acid were also investigated. Optimum conditions for the thiol labeling reaction have been investigated using the parameters of pH, buffer type, time, temperature, and relative concentrations. Under appropriate conditions the fluorescence produced by the reaction of ammonium 4-chloro-7-sulfobenzofurazan is linearly related to thiol concentration. The fluorescence intensity of 7-sulfobenzofurazan thiol derivatives are considerably greater than the corresponding 7-nitrobenzofurazan derivatives in both aqueous and aprotic solvents, rendering this reagent highly sensitive. Our preliminary experiments with proteins labeled with ammonium 4-chloro-7-sulfobenzofurazan have shown the probe to be removable by thiolysis with excess 2-mercaptoethanol." @default.
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- W2048770748 title "Ammonium 4-chloro-7-sulfobenzofurazan: A new fluorigenic thiol-specific reagent" @default.
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- W2048770748 doi "https://doi.org/10.1016/0003-9861(82)90043-1" @default.
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