FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048798052> ?p ?o ?g. }

• W2048798052 endingPage "432" @default.
• W2048798052 startingPage "427" @default.
• W2048798052 abstract "Objective To evaluate the ability of trastuzumab (Herceptin) Fab, labelled with 99mTc through introduced hydrazinenicotinamide (HYNIC) functionalities, to image HER2/neu-overexpressing human breast cancer xenografts in athymic mice. Methods Fab fragments were produced by immobilized papain digestion of trastuzumab immunoglobulin G (IgG), followed by purification by ultrafiltration. The immunoreactivity of trastuzumab Fab was evaluated by receptor-binding assays against HER2/neu-positive SK-BR-3 human breast cancer cells. Trastuzumab Fab fragments were labelled with 99mTc following modification with HYNIC N-hydroxysuccinimide ester. Biodistribution and tumour imaging studies were performed in athymic mice bearing subcutaneous HER2/neu-overexpressing BT-474 human breast cancer xenografts following intravenous injection of 1.1 or 25 MBq of [99mTc]-trastuzumab Fab (30 μg), respectively. The specificity of tumour uptake was assessed by comparison with that of [99mTc]-labelled irrelevant anti-CD33 HuM195 Fab. Results Trastuzumab Fab was pure and exhibited preserved immunoreactivity towards SK-BR-3 cells (Kd=1.6×10−8 M). Modification with HYNIC diminished its receptor-binding affinity fourfold. [99mTc]-trastuzumab Fab localized avidly and specifically in BT-474 xenografts, achieving a tumour uptake of 10.7% of the injected dose (ID) per gram and a tumour to blood (T/B) ratio of 3 : 1 at 24 h. The tumour uptake and T/B ratio for [99mTc]-trastuzumab Fab were significantly higher than those for control [99mTc]-HuM195 Fab (2.6% ID · g−1 and 0.9 : 1, respectively; P<0.05). Tumours were imaged as early as 2 h post-injection of [99mTc]-trastuzumab Fab, but were more clearly visualized at 6 and 24 h post-injection. Conclusions [99mTc]-HYNIC-trastuzumab Fab localized specifically in HER2/neu-overexpressing human breast cancer xenografts in athymic mice, allowing imaging of the tumours within the useful lifetime of the radionuclide." @default.
• W2048798052 created "2016-06-24" @default.
• W2048798052 creator A5013995512 @default.
• W2048798052 creator A5023465589 @default.
• W2048798052 creator A5033386419 @default.
• W2048798052 creator A5045329246 @default.
• W2048798052 creator A5070375413 @default.
• W2048798052 creator A5072531984 @default.
• W2048798052 date "2005-05-01" @default.
• W2048798052 modified "2023-09-25" @default.
• W2048798052 title "Imaging of HER2/neu expression in BT-474 human breast cancer xenografts in athymic mice using [99mTc]-HYNIC-trastuzumab (Herceptin) Fab fragments" @default.
• W2048798052 cites W1574013273 @default.
• W2048798052 cites W1837870834 @default.
• W2048798052 cites W1973033337 @default.
• W2048798052 cites W1974166931 @default.
• W2048798052 cites W1986967984 @default.
• W2048798052 cites W2006016180 @default.
• W2048798052 cites W2006174096 @default.
• W2048798052 cites W2024651005 @default.
• W2048798052 cites W2028534390 @default.
• W2048798052 cites W2030040451 @default.
• W2048798052 cites W2082717401 @default.
• W2048798052 cites W2094627540 @default.
• W2048798052 cites W2095156669 @default.
• W2048798052 cites W2108182027 @default.
• W2048798052 cites W2125730396 @default.
• W2048798052 cites W2126620646 @default.
• W2048798052 cites W2127268764 @default.
• W2048798052 cites W2136166098 @default.
• W2048798052 cites W2168631900 @default.
• W2048798052 cites W2318605753 @default.
• W2048798052 cites W4239989216 @default.
• W2048798052 cites W9050420 @default.
• W2048798052 doi "https://doi.org/10.1097/00006231-200505000-00006" @default.
• W2048798052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15838425" @default.
• W2048798052 hasPublicationYear "2005" @default.
• W2048798052 type Work @default.
• W2048798052 sameAs 2048798052 @default.
• W2048798052 citedByCount "60" @default.
• W2048798052 countsByYear W20487980522012 @default.
• W2048798052 countsByYear W20487980522013 @default.
• W2048798052 countsByYear W20487980522014 @default.
• W2048798052 countsByYear W20487980522016 @default.
• W2048798052 countsByYear W20487980522017 @default.
• W2048798052 countsByYear W20487980522018 @default.
• W2048798052 countsByYear W20487980522019 @default.
• W2048798052 countsByYear W20487980522020 @default.
• W2048798052 countsByYear W20487980522021 @default.
• W2048798052 countsByYear W20487980522022 @default.
• W2048798052 countsByYear W20487980522023 @default.
• W2048798052 crossrefType "journal-article" @default.
• W2048798052 hasAuthorship W2048798052A5013995512 @default.
• W2048798052 hasAuthorship W2048798052A5023465589 @default.
• W2048798052 hasAuthorship W2048798052A5033386419 @default.
• W2048798052 hasAuthorship W2048798052A5045329246 @default.
• W2048798052 hasAuthorship W2048798052A5070375413 @default.
• W2048798052 hasAuthorship W2048798052A5072531984 @default.
• W2048798052 hasConcept C121608353 @default.
• W2048798052 hasConcept C126322002 @default.
• W2048798052 hasConcept C153911025 @default.
• W2048798052 hasConcept C159654299 @default.
• W2048798052 hasConcept C185592680 @default.
• W2048798052 hasConcept C202751555 @default.
• W2048798052 hasConcept C203014093 @default.
• W2048798052 hasConcept C2776872082 @default.
• W2048798052 hasConcept C2777807558 @default.
• W2048798052 hasConcept C2779786085 @default.
• W2048798052 hasConcept C530470458 @default.
• W2048798052 hasConcept C55493867 @default.
• W2048798052 hasConcept C71924100 @default.
• W2048798052 hasConcept C86803240 @default.
• W2048798052 hasConceptScore W2048798052C121608353 @default.
• W2048798052 hasConceptScore W2048798052C126322002 @default.
• W2048798052 hasConceptScore W2048798052C153911025 @default.
• W2048798052 hasConceptScore W2048798052C159654299 @default.
• W2048798052 hasConceptScore W2048798052C185592680 @default.
• W2048798052 hasConceptScore W2048798052C202751555 @default.
• W2048798052 hasConceptScore W2048798052C203014093 @default.
• W2048798052 hasConceptScore W2048798052C2776872082 @default.
• W2048798052 hasConceptScore W2048798052C2777807558 @default.
• W2048798052 hasConceptScore W2048798052C2779786085 @default.
• W2048798052 hasConceptScore W2048798052C530470458 @default.
• W2048798052 hasConceptScore W2048798052C55493867 @default.
• W2048798052 hasConceptScore W2048798052C71924100 @default.
• W2048798052 hasConceptScore W2048798052C86803240 @default.
• W2048798052 hasIssue "5" @default.
• W2048798052 hasLocation W20487980521 @default.
• W2048798052 hasLocation W20487980522 @default.
• W2048798052 hasOpenAccess W2048798052 @default.
• W2048798052 hasPrimaryLocation W20487980521 @default.
• W2048798052 hasRelatedWork W2085370843 @default.
• W2048798052 hasRelatedWork W2122924947 @default.
• W2048798052 hasRelatedWork W2130870133 @default.
• W2048798052 hasRelatedWork W2748952813 @default.
• W2048798052 hasRelatedWork W2899084033 @default.
• W2048798052 hasRelatedWork W3012061994 @default.
• W2048798052 hasRelatedWork W310430870 @default.
• W2048798052 hasRelatedWork W4255386419 @default.

• next