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- W2048811023 abstract "A single donor–acceptor (D–A) pair with a fluctuating FRET rate F is studied theoretically in the frame of a “two-state model” in which the FRET rate F fluctuates taking two values, F1 and F2, with average rates B for the forward and b for the backward transitions. Theoretical expressions are derived for the autocorrelation function g(2)DA(τ) and for the Mandel parameter QD,A(τ) allowing for background emission. Fluctuating intensities ID,A(t) and FRET efficiency E(t) = IA(t)/[IA(t) + ID(t)] are calculated with the help of the Monte Carlo technique. The probability wD,AN(T) of finding N photons in a time interval T, and the distribution of the FRET efficiency P(E) are found by statistical treating of the fluctuating intensities ID,A(t). The shape of the distribution wD,AN(T) enables one to find the values of the parameters: F1, F2, and b/B. The influence of the background light on g(2)DA(τ), QD,A(τ) and wD,AN(T) is studied. It is shown how the background light influences the ratio b/B found from the analysis of wD,AN(T) and P(E)." @default.
- W2048811023 created "2016-06-24" @default.
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- W2048811023 date "2013-01-01" @default.
- W2048811023 modified "2023-09-26" @default.
- W2048811023 title "Conformational changes in complex macromolecules studied by single donor–acceptor pair fluorescence" @default.
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- W2048811023 doi "https://doi.org/10.1039/c3cp51464a" @default.
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