FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048816400> ?p ?o ?g. }

• W2048816400 endingPage "1191" @default.
• W2048816400 startingPage "1179" @default.
• W2048816400 abstract "The transcriptional inhibitor 5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole (DRB) is an adenosine analog that has been shown to cause premature transcriptional termination and thus has been a useful tool to identify factors important for transcriptional elongation. Here, we establish an efficient system for studying DRB-sensitive steps of transcriptional elongation. In addition, we establish two novel effects of DRB not previously reported: intron stabilization and the induction of long transcripts by a mechanism other than premature termination. We found that DRB had a biphasic effect on T-cell receptor-beta (TCRbeta) transcripts driven by a tetracycline (tet)-responsive promoter in transfected HeLa cells. In the first phase, DRB caused a rapid decrease (within five minutes) of pre-mRNA and its spliced intron (IVS1(Cbeta1)), consistent with the known ability of DRB to inhibit transcription. In the second phase (which began ten minutes to two hours after treatment, depending on the dose), DRB dramatically increased the levels of IVS1(Cbeta1)-containing transcripts by a mechanism requiring de novo RNA synthesis. DRB induced the appearance of short 0.4 to 0.8 kb TCRbeta transcripts in vivo, indicating DRB enhances premature transcriptional termination. A approximately 475 nt prematurely terminated transcript (PT) was characterized that terminated at an internal poly(A) tract in the intron IVS1(Cbeta1). We identified three other effects of DRB. First, we observed that DRB induced the appearance of heterodisperse TCRbeta transcripts that were too long ( approximately 1 kb to >8 kb) to result from the type of premature termination events previously described. Their production was not promoter-specific, as we found that long transcripts were induced by DRB from both the tet-responsive and beta-actin promoters. Second, DRB upregulated full-length normal-sized c-myc mRNA, which provided further evidence that DRB has effects besides regulation of premature termination. Third, DRB stabilized lariat forms of the intron IVS1(Cbeta1), indicating that DRB exerts post-transcriptional actions. We propose that our model system will be useful for elucidating the factors that regulate RNA decay and transcriptional elongation in vivo." @default.
• W2048816400 created "2016-06-24" @default.
• W2048816400 creator A5028641640 @default.
• W2048816400 creator A5086056927 @default.
• W2048816400 date "2000-06-01" @default.
• W2048816400 modified "2023-10-16" @default.
• W2048816400 title "Rapid induction of nuclear transcripts and inhibition of intron decay in response to the polymerase II inhibitor DRB" @default.
• W2048816400 cites W1268830682 @default.
• W2048816400 cites W131591417 @default.
• W2048816400 cites W1484532115 @default.
• W2048816400 cites W1485746636 @default.
• W2048816400 cites W1572241925 @default.
• W2048816400 cites W1577893399 @default.
• W2048816400 cites W1589968725 @default.
• W2048816400 cites W1677175288 @default.
• W2048816400 cites W1883899565 @default.
• W2048816400 cites W1965977492 @default.
• W2048816400 cites W1971336145 @default.
• W2048816400 cites W1973000551 @default.
• W2048816400 cites W1975797969 @default.
• W2048816400 cites W1979455742 @default.
• W2048816400 cites W1981531064 @default.
• W2048816400 cites W1984064054 @default.
• W2048816400 cites W1991055245 @default.
• W2048816400 cites W1991985869 @default.
• W2048816400 cites W1995737813 @default.
• W2048816400 cites W2001406822 @default.
• W2048816400 cites W2002794419 @default.
• W2048816400 cites W2003168089 @default.
• W2048816400 cites W2008167615 @default.
• W2048816400 cites W2013043744 @default.
• W2048816400 cites W2017050402 @default.
• W2048816400 cites W2020329201 @default.
• W2048816400 cites W2022093371 @default.
• W2048816400 cites W2022787977 @default.
• W2048816400 cites W2033280619 @default.
• W2048816400 cites W2033866215 @default.
• W2048816400 cites W2035597391 @default.
• W2048816400 cites W2038724489 @default.
• W2048816400 cites W2040422211 @default.
• W2048816400 cites W2043382556 @default.
• W2048816400 cites W2044339550 @default.
• W2048816400 cites W2044386270 @default.
• W2048816400 cites W2048463752 @default.
• W2048816400 cites W2058858849 @default.
• W2048816400 cites W2071342923 @default.
• W2048816400 cites W2073601378 @default.
• W2048816400 cites W2074300083 @default.
• W2048816400 cites W2076100163 @default.
• W2048816400 cites W2082658854 @default.
• W2048816400 cites W2089559330 @default.
• W2048816400 cites W2090429649 @default.
• W2048816400 cites W2093633115 @default.
• W2048816400 cites W2103602818 @default.
• W2048816400 cites W2104820327 @default.
• W2048816400 cites W2108422586 @default.
• W2048816400 cites W2110680694 @default.
• W2048816400 cites W2118840469 @default.
• W2048816400 cites W2120619605 @default.
• W2048816400 cites W2125150600 @default.
• W2048816400 cites W2133279102 @default.
• W2048816400 cites W2138647063 @default.
• W2048816400 cites W2148330261 @default.
• W2048816400 cites W2148917483 @default.
• W2048816400 cites W2153973987 @default.
• W2048816400 cites W2161932414 @default.
• W2048816400 cites W2162181283 @default.
• W2048816400 cites W2162772056 @default.
• W2048816400 cites W2164163807 @default.
• W2048816400 cites W2165888176 @default.
• W2048816400 cites W2167073094 @default.
• W2048816400 cites W40051044 @default.
• W2048816400 cites W4234507044 @default.
• W2048816400 cites W4240862133 @default.
• W2048816400 cites W4248192375 @default.
• W2048816400 cites W4250465115 @default.
• W2048816400 cites W4255118745 @default.
• W2048816400 cites W4255937594 @default.
• W2048816400 doi "https://doi.org/10.1006/jmbi.2000.3745" @default.
• W2048816400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10873444" @default.
• W2048816400 hasPublicationYear "2000" @default.
• W2048816400 type Work @default.
• W2048816400 sameAs 2048816400 @default.
• W2048816400 citedByCount "28" @default.
• W2048816400 countsByYear W20488164002012 @default.
• W2048816400 countsByYear W20488164002013 @default.
• W2048816400 countsByYear W20488164002014 @default.
• W2048816400 countsByYear W20488164002015 @default.
• W2048816400 countsByYear W20488164002018 @default.
• W2048816400 countsByYear W20488164002020 @default.
• W2048816400 countsByYear W20488164002021 @default.
• W2048816400 crossrefType "journal-article" @default.
• W2048816400 hasAuthorship W2048816400A5028641640 @default.
• W2048816400 hasAuthorship W2048816400A5086056927 @default.
• W2048816400 hasConcept C101762097 @default.
• W2048816400 hasConcept C104317684 @default.
• W2048816400 hasConcept C105580179 @default.
• W2048816400 hasConcept C138885662 @default.

• next