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- W2048840822 abstract "A practical and simple procedure was developed for the analysis of lysergic acid diethylamide (LSD) in blood samples utilizing solid phase extraction isolute HCX column. The analysis was performed on a reversed phase HPLC ODS-Hypersil column, eluted by a mobile phase of 0.3% triethylamine in 68:32 phosphate buffer (0. I M, pH 8.0):acetonitrile. LSD eluates were measured on a fluorescence detector fixed at excitation and emission wavelengths of 303 and 413 nm respectively. Calibration curves of LSD were linear over the range of 0.2–5 ng/ml with a correlation coefficient of 0.99. The method was reproducible with inter- and intra-run coefficient of variation of 4.3 to 9.1 % respectively. The lower detection limit was about 0.2 ng/ml for spiked blood samples. The method was applied on authentic post-mortem and Road Traffic Act blood samples to confirm LSD-radioimmunoassay results. The method was found to be sensitive, rapid, reproducible and usable for routine forensic toxicology practice." @default.
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- W2048840822 date "1996-01-01" @default.
- W2048840822 modified "2023-09-24" @default.
- W2048840822 title "Analysis of lysergic acid diethylamide in whole blood by HPLC-fluorescence detection" @default.
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- W2048840822 doi "https://doi.org/10.3109/14659899609084994" @default.
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