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- W2048882097 abstract "Porphyromonas gingivalis is an oral pathogen linked to chronic periodontitis. The bacterium exists as part of a polymicrobial biofilm accreted onto the tooth surface. An understanding of the changes to the proteome especially of the cell envelope of biofilm cells compared with planktonic cells could provide valuable insight into the molecular processes of biofilm formation. To establish which proteins changed in abundance between the planktonic and biofilm growth states, the cell envelope fractions of two biological replicates of P. gingivalis cultivated in a chemostat were analysed. Proteins were separated by 1-D SDS-PAGE, in-gel digested with trypsin in the presence of H216O or H218O and identified and quantified by LC-MALDI TOF/TOF-MS. Using a reverse labeling strategy we identified and quantified the changes in abundance of 81 P. gingivalis cell envelope proteins. No form of bias between the labels was observed. Twenty four proteins increased in abundance and 18 decreased in abundance in the biofilm state. A group of cell-surface located C-Terminal Domain family proteins including RgpA, HagA, CPG70 and PG99 increased in abundance in the biofilm cells. Other proteins that exhibited significant changes in abundance included transport related proteins (HmuY and IhtB), metabolic enzymes (FrdAB) and immunogenic proteins." @default.
- W2048882097 created "2016-06-24" @default.
- W2048882097 creator A5005897817 @default.
- W2048882097 creator A5048673467 @default.
- W2048882097 creator A5066199522 @default.
- W2048882097 creator A5089222354 @default.
- W2048882097 date "2008-04-01" @default.
- W2048882097 modified "2023-10-10" @default.
- W2048882097 title "Application of16O/18O reverse proteolytic labeling to determine the effect of biofilm culture on the cell envelope proteome ofPorphyromonas gingivalis W50" @default.
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- W2048882097 doi "https://doi.org/10.1002/pmic.200700557" @default.
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