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- W2048896370 abstract "The proposal that the stable expression of organogenesis-directing plasma membrane antigens, such as testis-organizing H-Y antigen, requires β2-microglobulin-MHC antigen dimers as anchorage sites was tested on Daudi human Burkitt lymphoma cells [46, XY, 15q−, 14q+, β2-m(−), HLA(−)]. The H-Y antigen level of Daudi was only 20% of that of Raji and Ramos, two human male pseudodiploid Burkitt lymphoma lines that were β2-m(+), HLA(+). When Daudi is hybridized with β2-m(+), HLA(+) cell lines, β2-microglobulin, supplied by the latter, is known to restore the expression of Daudi HLA antigens A10 and BW17. Such restoration of HLA antigen expression markedly elevated H-Y antigen levels in those somatic hybrids. Thus the H-Y antigen level of the Daudi × Raji 8A (♂ × ♂) hybrid became equal to that of TetraRaji—the colcemide-induced Raji tetraploid line. Two independently derived Daudi × HeLa D98 (♂ × ♀) hybrids, DAD 1 and DAD 10, demonstrated even higher H-Y antigen levels comparable to that of normal male peripheral blood lymphocytes." @default.
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- W2048896370 date "1978-03-01" @default.
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- W2048896370 title "The HLA-dependent expression of testis-organizing H-Y antigen by human male cells" @default.
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- W2048896370 doi "https://doi.org/10.1016/0092-8674(78)90324-0" @default.
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