Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048930070> ?p ?o ?g. }
- W2048930070 endingPage "181" @default.
- W2048930070 startingPage "167" @default.
- W2048930070 abstract "Two major protein phosphatase (PP) activities were purified from cytosolic extracts of the erythrocytic stage of the malaria parasite, Plasmodium falciparum. Both enzymes were specific for phosphoserine and phosphothreonine residues with very little activity against phosphotyrosine residues. The biochemical properties of the enzymes suggested their strong similarity with eukaryotic PP2A and PP2B protein phosphatases. Both enzymes preferentially dephosphorylated the alpha subunit of phosphorylase kinase, and were resistant to inhibitor-1. The PP2A-like enzyme required Mn2+ for activity and was inhibited by nanomolar concentrations of okadaic acid (OA). The cDNA sequence of the PP2A-like enzyme was identified through a match of its predicted amino acid sequence with the N-terminal sequence of the catalytic subunit. The PP2B-like (calcineurin) enzyme was stimulated by calmodulin and Ca2+ or Ni2+, but was resistant to OA. Malarial calcineurin was strongly and specifically inhibited by cyclosporin A (CsA) only in the presence of wild type P. falciparum cyclophilin but not a mutant cyclophilin. The inhibition was noncompetitive, and provides a potential explanation for the cyclosporin-sensitivity of the parasite. There was no significant quantitative difference in the total protein Ser/Thr phosphatase activity among the ring, trophozoite, and schizont stages." @default.
- W2048930070 created "2016-06-24" @default.
- W2048930070 creator A5007213279 @default.
- W2048930070 creator A5026207705 @default.
- W2048930070 creator A5038753195 @default.
- W2048930070 creator A5050852618 @default.
- W2048930070 creator A5059969933 @default.
- W2048930070 creator A5060850866 @default.
- W2048930070 creator A5090453645 @default.
- W2048930070 date "1999-04-01" @default.
- W2048930070 modified "2023-10-16" @default.
- W2048930070 title "Characterization of protein Ser/Thr phosphatases of the malaria parasite, Plasmodium falciparum: inhibition of the parasitic calcineurin by cyclophilin-cyclosporin complex" @default.
- W2048930070 cites W1568494138 @default.
- W2048930070 cites W1739306715 @default.
- W2048930070 cites W1892570697 @default.
- W2048930070 cites W1956619778 @default.
- W2048930070 cites W1958477972 @default.
- W2048930070 cites W1967275231 @default.
- W2048930070 cites W1972172786 @default.
- W2048930070 cites W1972455374 @default.
- W2048930070 cites W1981784970 @default.
- W2048930070 cites W1982388650 @default.
- W2048930070 cites W1984470094 @default.
- W2048930070 cites W1984947099 @default.
- W2048930070 cites W198565253 @default.
- W2048930070 cites W1993946246 @default.
- W2048930070 cites W1997939560 @default.
- W2048930070 cites W2003746309 @default.
- W2048930070 cites W2025395236 @default.
- W2048930070 cites W2027226278 @default.
- W2048930070 cites W2030126092 @default.
- W2048930070 cites W2031872230 @default.
- W2048930070 cites W2033307739 @default.
- W2048930070 cites W2034031307 @default.
- W2048930070 cites W2036116153 @default.
- W2048930070 cites W2039215369 @default.
- W2048930070 cites W2042377147 @default.
- W2048930070 cites W2044690468 @default.
- W2048930070 cites W2045987477 @default.
- W2048930070 cites W2047835322 @default.
- W2048930070 cites W2053645188 @default.
- W2048930070 cites W2058029113 @default.
- W2048930070 cites W2068348400 @default.
- W2048930070 cites W2069964674 @default.
- W2048930070 cites W2080915609 @default.
- W2048930070 cites W2081178976 @default.
- W2048930070 cites W2091136241 @default.
- W2048930070 cites W2097441294 @default.
- W2048930070 cites W2100837269 @default.
- W2048930070 cites W2129196156 @default.
- W2048930070 cites W2130592046 @default.
- W2048930070 cites W2137221055 @default.
- W2048930070 cites W2140707292 @default.
- W2048930070 cites W2153405970 @default.
- W2048930070 cites W2156339161 @default.
- W2048930070 cites W2158608067 @default.
- W2048930070 cites W223364330 @default.
- W2048930070 cites W252703155 @default.
- W2048930070 cites W4211158748 @default.
- W2048930070 cites W4244850859 @default.
- W2048930070 doi "https://doi.org/10.1016/s0166-6851(99)00010-9" @default.
- W2048930070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10340482" @default.
- W2048930070 hasPublicationYear "1999" @default.
- W2048930070 type Work @default.
- W2048930070 sameAs 2048930070 @default.
- W2048930070 citedByCount "60" @default.
- W2048930070 countsByYear W20489300702012 @default.
- W2048930070 countsByYear W20489300702013 @default.
- W2048930070 countsByYear W20489300702014 @default.
- W2048930070 countsByYear W20489300702015 @default.
- W2048930070 countsByYear W20489300702016 @default.
- W2048930070 countsByYear W20489300702017 @default.
- W2048930070 countsByYear W20489300702022 @default.
- W2048930070 countsByYear W20489300702023 @default.
- W2048930070 crossrefType "journal-article" @default.
- W2048930070 hasAuthorship W2048930070A5007213279 @default.
- W2048930070 hasAuthorship W2048930070A5026207705 @default.
- W2048930070 hasAuthorship W2048930070A5038753195 @default.
- W2048930070 hasAuthorship W2048930070A5050852618 @default.
- W2048930070 hasAuthorship W2048930070A5059969933 @default.
- W2048930070 hasAuthorship W2048930070A5060850866 @default.
- W2048930070 hasAuthorship W2048930070A5090453645 @default.
- W2048930070 hasConcept C104292427 @default.
- W2048930070 hasConcept C104317684 @default.
- W2048930070 hasConcept C128057223 @default.
- W2048930070 hasConcept C141071460 @default.
- W2048930070 hasConcept C153911025 @default.
- W2048930070 hasConcept C167625842 @default.
- W2048930070 hasConcept C178666793 @default.
- W2048930070 hasConcept C181199279 @default.
- W2048930070 hasConcept C203014093 @default.
- W2048930070 hasConcept C2776326521 @default.
- W2048930070 hasConcept C2776414213 @default.
- W2048930070 hasConcept C2776953658 @default.
- W2048930070 hasConcept C2778048844 @default.
- W2048930070 hasConcept C2778371730 @default.
- W2048930070 hasConcept C2780307904 @default.
- W2048930070 hasConcept C2911091166 @default.