FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048931146> ?p ?o ?g. }

• W2048931146 endingPage "6608" @default.
• W2048931146 startingPage "6603" @default.
• W2048931146 abstract "Edwardsiella tarda is the etiological agent of edwardsiellosis, a systematic disease that affects a wide range of marine and freshwater fish cultured worldwide. In order to identify E. tarda antigens with vaccine potential, we in this study conducted a systematic search for E. tarda proteins with secretion capacity. One of the proteins thus identified was Esa1, which contains 795 amino acid residues and shares extensive overall sequence identities with the D15-like surface antigens of several bacterial species. In silico analyses indicated that Esa1 localizes to outer membrane and possesses domain structures that are conserved among bacterial surface antigens. The vaccine potential of purified recombinant Esa1 was examined in a Japanese flounder (Paralichthys olivaceus) model, which showed that fish vaccinated with Esa1 exhibited a high level of survival and produced specific serum antibodies. Passive immunization of naïve fish with antisera raised against Esa1 resulted in significant protection against E. tarda challenge. Taking advantage of the secretion capacity of Esa1 and the natural gut-colonization ability of a fish commensal strain, we constructed an Esa1-expressing recombinant strain, FP3/pJsa1. Western immunoblot and agglutination analyses showed that FP3/pJsa1 produces outer membrane-localized Esa1 and forms aggregates in the presence of anti-Esa1 antibodies. Vaccination analyses showed that FP3/pJsa1 as an intraperitoneal injection vaccine and an oral vaccine embedded in alginate microspheres produced relative percent survival rates of 79% and 52%, respectively, under severe challenging conditions that resulted in 92-96% mortality in control fish. Further analyses showed that following oral vaccination, FP3/pJsa1 was able to colonize in the gut but unable to disseminate into other tissues. Together these results indicate that Esa1 is a protective immunogen and an effective oral vaccine when delivered by FP3/pJsa1 as a surface-anchored antigen." @default.
• W2048931146 created "2016-06-24" @default.
• W2048931146 creator A5029763941 @default.
• W2048931146 creator A5049415248 @default.
• W2048931146 creator A5060187327 @default.
• W2048931146 date "2010-09-01" @default.
• W2048931146 modified "2023-09-27" @default.
• W2048931146 title "Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain" @default.
• W2048931146 cites W1562263727 @default.
• W2048931146 cites W1849156717 @default.
• W2048931146 cites W1972386757 @default.
• W2048931146 cites W1981960942 @default.
• W2048931146 cites W1984167965 @default.
• W2048931146 cites W1984174034 @default.
• W2048931146 cites W1985973479 @default.
• W2048931146 cites W1989492617 @default.
• W2048931146 cites W1990177413 @default.
• W2048931146 cites W1992495958 @default.
• W2048931146 cites W1999721004 @default.
• W2048931146 cites W2002725057 @default.
• W2048931146 cites W2016854182 @default.
• W2048931146 cites W2025187218 @default.
• W2048931146 cites W2026111634 @default.
• W2048931146 cites W2038276497 @default.
• W2048931146 cites W2039505572 @default.
• W2048931146 cites W2039737435 @default.
• W2048931146 cites W2041234609 @default.
• W2048931146 cites W2044167045 @default.
• W2048931146 cites W2059611232 @default.
• W2048931146 cites W2060166908 @default.
• W2048931146 cites W2061296073 @default.
• W2048931146 cites W2069096191 @default.
• W2048931146 cites W2072282717 @default.
• W2048931146 cites W2077691179 @default.
• W2048931146 cites W2085102105 @default.
• W2048931146 cites W2090182678 @default.
• W2048931146 cites W2111382437 @default.
• W2048931146 cites W2120032128 @default.
• W2048931146 cites W2120145681 @default.
• W2048931146 cites W2124866210 @default.
• W2048931146 cites W2134840969 @default.
• W2048931146 cites W2135322944 @default.
• W2048931146 cites W2153052261 @default.
• W2048931146 cites W2155349840 @default.
• W2048931146 doi "https://doi.org/10.1016/j.vaccine.2010.07.050" @default.
• W2048931146 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20673823" @default.
• W2048931146 hasPublicationYear "2010" @default.
• W2048931146 type Work @default.
• W2048931146 sameAs 2048931146 @default.
• W2048931146 citedByCount "41" @default.
• W2048931146 countsByYear W20489311462012 @default.
• W2048931146 countsByYear W20489311462013 @default.
• W2048931146 countsByYear W20489311462014 @default.
• W2048931146 countsByYear W20489311462015 @default.
• W2048931146 countsByYear W20489311462016 @default.
• W2048931146 countsByYear W20489311462017 @default.
• W2048931146 countsByYear W20489311462018 @default.
• W2048931146 countsByYear W20489311462019 @default.
• W2048931146 countsByYear W20489311462020 @default.
• W2048931146 countsByYear W20489311462021 @default.
• W2048931146 countsByYear W20489311462022 @default.
• W2048931146 countsByYear W20489311462023 @default.
• W2048931146 crossrefType "journal-article" @default.
• W2048931146 hasAuthorship W2048931146A5029763941 @default.
• W2048931146 hasAuthorship W2048931146A5049415248 @default.
• W2048931146 hasAuthorship W2048931146A5060187327 @default.
• W2048931146 hasConcept C104317684 @default.
• W2048931146 hasConcept C147483822 @default.
• W2048931146 hasConcept C159047783 @default.
• W2048931146 hasConcept C159654299 @default.
• W2048931146 hasConcept C203014093 @default.
• W2048931146 hasConcept C22070199 @default.
• W2048931146 hasConcept C2776533291 @default.
• W2048931146 hasConcept C2776832872 @default.
• W2048931146 hasConcept C2909208804 @default.
• W2048931146 hasConcept C40767141 @default.
• W2048931146 hasConcept C505870484 @default.
• W2048931146 hasConcept C55493867 @default.
• W2048931146 hasConcept C86803240 @default.
• W2048931146 hasConcept C89423630 @default.
• W2048931146 hasConceptScore W2048931146C104317684 @default.
• W2048931146 hasConceptScore W2048931146C147483822 @default.
• W2048931146 hasConceptScore W2048931146C159047783 @default.
• W2048931146 hasConceptScore W2048931146C159654299 @default.
• W2048931146 hasConceptScore W2048931146C203014093 @default.
• W2048931146 hasConceptScore W2048931146C22070199 @default.
• W2048931146 hasConceptScore W2048931146C2776533291 @default.
• W2048931146 hasConceptScore W2048931146C2776832872 @default.
• W2048931146 hasConceptScore W2048931146C2909208804 @default.
• W2048931146 hasConceptScore W2048931146C40767141 @default.
• W2048931146 hasConceptScore W2048931146C505870484 @default.
• W2048931146 hasConceptScore W2048931146C55493867 @default.
• W2048931146 hasConceptScore W2048931146C86803240 @default.
• W2048931146 hasConceptScore W2048931146C89423630 @default.
• W2048931146 hasIssue "40" @default.
• W2048931146 hasLocation W20489311461 @default.
• W2048931146 hasLocation W20489311462 @default.
• W2048931146 hasOpenAccess W2048931146 @default.

• next