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• W2048932008 abstract "To identify an in vitro culture procedure which can be used to assess porcine in vitro fertilized embryos, three culture systems were examined. A total of 103 in vivo fertilized zygotes were co-cultured in modified BMOC-3 medium with granulosa cells, or in modified BMOC-2 with oviduct cells, and 35 were cultured in Whitten's medium supplemented with 1.5% BSA. After 8 days in culture, 66.0 and 73.6% of the co-cultured zygotes, and 51.4% of the zygotes cultured in the modified Whitten's medium developed to blastocyst stage. In contrast, only 20.5% (18/88) and 1.4% (9/627) of in vitro fertilized zygotes derived from in vivo and in vitro matured oocytes developed to blastocysts in the co-culture with modified BMOC-3 and granulosa cells. It is concluded that the culture systems examined are competent to support development of porcine zygotes to blastocysts and evaluate in vitro fertilized porcine embryos." @default.
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• W2048932008 date "1993-01-01" @default.
• W2048932008 modified "2023-10-15" @default.
• W2048932008 title "In Vitro Development of in Vivo and in Vitro Fertilized Porcine Zygotes." @default.
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• W2048932008 doi "https://doi.org/10.1262/jrd.39.163" @default.
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