Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048971314> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2048971314 endingPage "1045" @default.
- W2048971314 startingPage "1037" @default.
- W2048971314 abstract "Two independent analytical methods for determining the activity and stability profile of liquid yeast derived sucrase (YS) were established and validated in order to conduct preliminary stability studies as a function of temperature. The methods included a hexokinase-based (HK) enzymatic assay for determining the formation of glucose upon hydrolysis of sucrose by YS, and a direct polarimetric procedure to quantitate YS hydrolysis of sucrose. Both assays were validated with respect to YS dilution, incubation time, sucrose or glucose concentration, linearity of response and within- and between-day variability. A preliminary stability study was conducted over a 24 week period with liquid YS samples stored at −20, 4, 30, 40 and 50°C. Enzymatic activity was monitored as a function of time using both the HK and polarimetric assays. Liquid YS samples stored at −20, 4 and 30°C retained 100% activity after 24 weeks storage, while the samples stored at 40°C lost approximately 70% activity over the same storage period and samples stored at 50°C lost approximately 95% activity after 12 weeks storage. The two methods of analysis gave consistent results over the course of the study." @default.
- W2048971314 created "2016-06-24" @default.
- W2048971314 creator A5006734746 @default.
- W2048971314 creator A5021731840 @default.
- W2048971314 creator A5067544706 @default.
- W2048971314 creator A5080162519 @default.
- W2048971314 date "1998-09-01" @default.
- W2048971314 modified "2023-10-13" @default.
- W2048971314 title "Analytical methods and stability assessment of liquid yeast derived sucrase" @default.
- W2048971314 cites W1506245646 @default.
- W2048971314 cites W1520035512 @default.
- W2048971314 cites W1524703060 @default.
- W2048971314 cites W189908257 @default.
- W2048971314 cites W2085489408 @default.
- W2048971314 cites W2086852022 @default.
- W2048971314 cites W2323689259 @default.
- W2048971314 cites W2332208375 @default.
- W2048971314 cites W2332475845 @default.
- W2048971314 doi "https://doi.org/10.1016/s0731-7085(98)00069-7" @default.
- W2048971314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9884193" @default.
- W2048971314 hasPublicationYear "1998" @default.
- W2048971314 type Work @default.
- W2048971314 sameAs 2048971314 @default.
- W2048971314 citedByCount "1" @default.
- W2048971314 countsByYear W20489713142013 @default.
- W2048971314 crossrefType "journal-article" @default.
- W2048971314 hasAuthorship W2048971314A5006734746 @default.
- W2048971314 hasAuthorship W2048971314A5021731840 @default.
- W2048971314 hasAuthorship W2048971314A5067544706 @default.
- W2048971314 hasAuthorship W2048971314A5080162519 @default.
- W2048971314 hasConcept C121332964 @default.
- W2048971314 hasConcept C181199279 @default.
- W2048971314 hasConcept C185592680 @default.
- W2048971314 hasConcept C2776039999 @default.
- W2048971314 hasConcept C2778597767 @default.
- W2048971314 hasConcept C2779222958 @default.
- W2048971314 hasConcept C43617362 @default.
- W2048971314 hasConcept C55493867 @default.
- W2048971314 hasConcept C94412978 @default.
- W2048971314 hasConcept C95986675 @default.
- W2048971314 hasConcept C96618451 @default.
- W2048971314 hasConcept C97355855 @default.
- W2048971314 hasConceptScore W2048971314C121332964 @default.
- W2048971314 hasConceptScore W2048971314C181199279 @default.
- W2048971314 hasConceptScore W2048971314C185592680 @default.
- W2048971314 hasConceptScore W2048971314C2776039999 @default.
- W2048971314 hasConceptScore W2048971314C2778597767 @default.
- W2048971314 hasConceptScore W2048971314C2779222958 @default.
- W2048971314 hasConceptScore W2048971314C43617362 @default.
- W2048971314 hasConceptScore W2048971314C55493867 @default.
- W2048971314 hasConceptScore W2048971314C94412978 @default.
- W2048971314 hasConceptScore W2048971314C95986675 @default.
- W2048971314 hasConceptScore W2048971314C96618451 @default.
- W2048971314 hasConceptScore W2048971314C97355855 @default.
- W2048971314 hasIssue "6-7" @default.
- W2048971314 hasLocation W20489713141 @default.
- W2048971314 hasLocation W20489713142 @default.
- W2048971314 hasOpenAccess W2048971314 @default.
- W2048971314 hasPrimaryLocation W20489713141 @default.
- W2048971314 hasRelatedWork W1197571916 @default.
- W2048971314 hasRelatedWork W181343588 @default.
- W2048971314 hasRelatedWork W1967508422 @default.
- W2048971314 hasRelatedWork W1968151762 @default.
- W2048971314 hasRelatedWork W2030944380 @default.
- W2048971314 hasRelatedWork W2036207985 @default.
- W2048971314 hasRelatedWork W2057120196 @default.
- W2048971314 hasRelatedWork W2321096530 @default.
- W2048971314 hasRelatedWork W2398001813 @default.
- W2048971314 hasRelatedWork W2546360479 @default.
- W2048971314 hasVolume "17" @default.
- W2048971314 isParatext "false" @default.
- W2048971314 isRetracted "false" @default.
- W2048971314 magId "2048971314" @default.
- W2048971314 workType "article" @default.