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- W2048987252 abstract "The two mouse melanoma cell lines B16-F1 and B16-G4F retain their melanogenic capacity when cultured in vitro. Melanotropic peptides such as alpha-melanocyte-stimulating hormone (alpha-MSH) induce formation and release of melanin pigment in B16-F1 cells. In contrast, B16-G4F cells do not respond to alpha-MSH. Using receptor-binding analysis and photoaffinity crosslinking we demonstrate that the lack of response of B16-G4F cells to alpha-MSH is due to the absence of functional MSH receptors from the cell surface. Northern blot analysis of receptor mRNA revealed that MSH receptor mRNA is not expressed in B16-G4F cells. These cells represent a new tool for the study of signal pathways related to the control of melanogenesis in melanoma cells." @default.
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- W2048987252 date "1993-05-10" @default.
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- W2048987252 title "B16‐G4F mouse melanoma cells: An MSH receptor‐deficient cell clone" @default.
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- W2048987252 doi "https://doi.org/10.1016/0014-5793(93)81563-f" @default.
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