FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2048993909> ?p ?o ?g. }

• W2048993909 endingPage "216" @default.
• W2048993909 startingPage "205" @default.
• W2048993909 abstract "We report the properties of the new AloI restriction and modification enzyme from Acinetobacter lwoffi Ks 4-8 that recognizes the DNA target 5' GGA(N)6GTTC3' (complementary strand 5' GAAC(N)6TCC3'), and the nucleotide sequence of the gene encoding this enzyme. AloI is a bifunctional large polypeptide (deduced M(r) 143 kDa) revealing both DNA endonuclease and methyltransferase activities. Depending on reaction cofactors, AloI cleaves double-stranded DNA on both strands, seven bases on the 5' side, and 12-13 bases on the 3' side of its recognition sequence, and modifies adenine residues in both DNA strands in the target sequence yielding N6-methyladenine. For cleavage activity AloI maintains an absolute requirement for Mg(2+) and does not depend on or is stimulated by either ATP or S-adenosyl-L-methionine. Modification function requires the presence of S-adenosyl-L-methionine and is stimulated by metal ions (Ca(2+)). The C-terminal and central parts of the protein were found to be homologous to certain specificity (HsdS) and modification (HsdM) subunits of type I R-M systems, respectively. The N-terminal part of the protein possesses the putative endonucleolytic motif DXnEXK of restriction endonucleases. The deduced amino acid sequence of AloI shares significant homology with polypeptides encoding HaeIV and CjeI restriction-modification proteins at the N-terminal and central, but not at the C-terminal domains. The organization of AloI implies that its evolution involved fusion of an endonuclease and the two subunits, HsdM and HsdS, of type I restriction enzymes. According to the structure and function properties AloI may be regarded as one more representative of a newly emerging group of HaeIV-like restriction endonucleases. Discovery of these enzymes opens new opportunities for constructing restriction endonucleases with a new specificity." @default.
• W2048993909 created "2016-06-24" @default.
• W2048993909 creator A5010435977 @default.
• W2048993909 creator A5016649181 @default.
• W2048993909 creator A5022379440 @default.
• W2048993909 creator A5036435762 @default.
• W2048993909 creator A5060013478 @default.
• W2048993909 creator A5073734787 @default.
• W2048993909 creator A5087477834 @default.
• W2048993909 date "2001-11-01" @default.
• W2048993909 modified "2023-10-18" @default.
• W2048993909 title "Characterization of AloI, a restriction-modification system of a new type" @default.
• W2048993909 cites W117297858 @default.
• W2048993909 cites W1568363084 @default.
• W2048993909 cites W1599794366 @default.
• W2048993909 cites W1738997773 @default.
• W2048993909 cites W1973380974 @default.
• W2048993909 cites W1981766010 @default.
• W2048993909 cites W1982315729 @default.
• W2048993909 cites W1990995746 @default.
• W2048993909 cites W2000236367 @default.
• W2048993909 cites W2002650712 @default.
• W2048993909 cites W2006501102 @default.
• W2048993909 cites W2007458839 @default.
• W2048993909 cites W2015292449 @default.
• W2048993909 cites W2015420917 @default.
• W2048993909 cites W2015593682 @default.
• W2048993909 cites W2019410656 @default.
• W2048993909 cites W2022335863 @default.
• W2048993909 cites W2024963213 @default.
• W2048993909 cites W2034079328 @default.
• W2048993909 cites W2034134994 @default.
• W2048993909 cites W2038124483 @default.
• W2048993909 cites W2038651584 @default.
• W2048993909 cites W2044721821 @default.
• W2048993909 cites W2047467752 @default.
• W2048993909 cites W2061035293 @default.
• W2048993909 cites W2061648185 @default.
• W2048993909 cites W2076796342 @default.
• W2048993909 cites W2078476027 @default.
• W2048993909 cites W2082402822 @default.
• W2048993909 cites W2085189454 @default.
• W2048993909 cites W2088710319 @default.
• W2048993909 cites W2100837269 @default.
• W2048993909 cites W2104356076 @default.
• W2048993909 cites W2109241103 @default.
• W2048993909 cites W2112669362 @default.
• W2048993909 cites W2134296866 @default.
• W2048993909 cites W2141221250 @default.
• W2048993909 cites W2144336085 @default.
• W2048993909 cites W2155159892 @default.
• W2048993909 cites W2171434257 @default.
• W2048993909 cites W2467450268 @default.
• W2048993909 cites W4243964386 @default.
• W2048993909 doi "https://doi.org/10.1006/jmbi.2001.5049" @default.
• W2048993909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11718555" @default.
• W2048993909 hasPublicationYear "2001" @default.
• W2048993909 type Work @default.
• W2048993909 sameAs 2048993909 @default.
• W2048993909 citedByCount "32" @default.
• W2048993909 countsByYear W20489939092012 @default.
• W2048993909 countsByYear W20489939092013 @default.
• W2048993909 countsByYear W20489939092014 @default.
• W2048993909 countsByYear W20489939092018 @default.
• W2048993909 crossrefType "journal-article" @default.
• W2048993909 hasAuthorship W2048993909A5010435977 @default.
• W2048993909 hasAuthorship W2048993909A5016649181 @default.
• W2048993909 hasAuthorship W2048993909A5022379440 @default.
• W2048993909 hasAuthorship W2048993909A5036435762 @default.
• W2048993909 hasAuthorship W2048993909A5060013478 @default.
• W2048993909 hasAuthorship W2048993909A5073734787 @default.
• W2048993909 hasAuthorship W2048993909A5087477834 @default.
• W2048993909 hasConcept C104317684 @default.
• W2048993909 hasConcept C128319531 @default.
• W2048993909 hasConcept C167625842 @default.
• W2048993909 hasConcept C181199279 @default.
• W2048993909 hasConcept C2777028655 @default.
• W2048993909 hasConcept C512185932 @default.
• W2048993909 hasConcept C552990157 @default.
• W2048993909 hasConcept C55493867 @default.
• W2048993909 hasConcept C86803240 @default.
• W2048993909 hasConceptScore W2048993909C104317684 @default.
• W2048993909 hasConceptScore W2048993909C128319531 @default.
• W2048993909 hasConceptScore W2048993909C167625842 @default.
• W2048993909 hasConceptScore W2048993909C181199279 @default.
• W2048993909 hasConceptScore W2048993909C2777028655 @default.
• W2048993909 hasConceptScore W2048993909C512185932 @default.
• W2048993909 hasConceptScore W2048993909C552990157 @default.
• W2048993909 hasConceptScore W2048993909C55493867 @default.
• W2048993909 hasConceptScore W2048993909C86803240 @default.
• W2048993909 hasIssue "2" @default.
• W2048993909 hasLocation W20489939091 @default.
• W2048993909 hasLocation W20489939092 @default.
• W2048993909 hasOpenAccess W2048993909 @default.
• W2048993909 hasPrimaryLocation W20489939091 @default.
• W2048993909 hasRelatedWork W1587466397 @default.
• W2048993909 hasRelatedWork W1964819917 @default.
• W2048993909 hasRelatedWork W1973671464 @default.

• next