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• W2048996915 abstract "A pepide nucleic acid (PNA) monomer containing the universal base 3-nitropyrrole was synthesized by coupling 1-carboxymethyl-3-nitropyrrole to ethyl N-[2-(tert-butoxycarbonylamino)ethyl]glycinate. The PNA sequence H-TGTACGTXACAACTA-NH2 (X = 3-nitropyrrole and C) and DNA sequence 5′-TGTACGTXACAACTA-3′ were synthesized and thermal melting studies with the complementary DNA sequence 5′-TAGTTGTYACGTACA-3′ (Y = A,C, G, T) compared. The Tm data show that 3-nitropyrrole pairs indiscriminately with all four natural nucleobases as a constituent of either DNA or PNA. However, 3-nitropyrrole-containing PNA-DNA (average Tm value = 51.1°C) is significantly more thermally stable than 3-nitropyrrole-containing DNA-DNA (average Tm value = 39.6°C). From circular dichroism measurements, it is apparent that 3-nitropyrrole in the PNA strand causes a significant change in duplex structure." @default.
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• W2048996915 date "2001-02-01" @default.
• W2048996915 modified "2023-09-22" @default.
• W2048996915 title "Peptide Nucleic Acid-DNA Duplexes Containing the Universal Base 3-Nitropyrrole" @default.
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• W2048996915 doi "https://doi.org/10.1006/meth.2000.1114" @default.
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