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- W2049012791 abstract "Abstract Gas vesicles provide buoyancy in many aquatic cyanobacteria and planktonic bacteria. An apparatus comprising three capillary compression tubes is described for the determination of the gas vesicle volume in a cell suspension. It provides replicate measurements of the irreversible volumetric contraction when gas vesicles are collapsed by pressure. Potential errors in the measurement associated with thermal instability, calibration of the tubes and capillaries, the formation of gas bubbles and the effects of surface tension are discussed. Highly reproducible measurements were obtained with suspensions containing as little as 0.5 μl ml−1 of gas vesicle gas. The use of the apparatus is described in quantitative studies of the critical collapse pressures of gas vesicles, changes in gas vesicle content of cell suspensions, the proportion of cell volume occupied by gas space, the proportion of the gas vesicles required for neutral buoyancy, and the buoyant density of floating cells. These measurements are required in quantitative studies on buoyancy regulation and waterbloom formation by cyanobacteria (blue-green algae)." @default.
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- W2049012791 date "1992-06-01" @default.
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- W2049012791 title "The measurement of gas vesicle volume and buoyant density in planktonic bacteria" @default.
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- W2049012791 doi "https://doi.org/10.1016/0167-7012(92)90048-9" @default.
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