FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049027654> ?p ?o ?g. }

• W2049027654 endingPage "15060" @default.
• W2049027654 startingPage "15052" @default.
• W2049027654 abstract "Gram-negative bacterial endotoxin (i.e. lipopolysaccharide (LPS)) is one of the most potent stimulants of the innate immune system, recognized by the TLR4.MD-2 complex. Direct binding to MD-2 of LPS and LPS analogues that act as TLR4 agonists or antagonists is well established, but the role of MD-2 and TLR4 in receptor activation is much less clear. We have identified residues within the hairpin of MD-2 between strands five and six that, although not contacting acyl chains of tetraacylated lipid IVa (a TLR4 antagonist), influence activation of TLR4 by hexaacylated lipid A. We show that hydrophobic residues at positions 82, 85, and 87 of MD-2 are essential both for transfer of endotoxin from CD14 to monomeric MD-2 and for TLR4 activation. We also identified a pair of conserved hydrophobic residues (Phe-440 and Phe-463) in leucine-rich repeats 16 and 17 of the TLR4 ectodomain, which are essential for activation of TLR4 by LPS. F440A or F463A mutants of TLR4 were inactive, whereas the F440W mutant retained full activity. Charge reversal of neighboring cationic groups in the TLR4 ectodomain (Lys-388 and Lys-435), in contrast, did not affect cell activation. Our mutagenesis studies are consistent with a molecular model in which Val-82, Met-85, and Leu-87 in MD-2 and distal portions of a secondary acyl chain of hexaacylated lipid A that do not fit into the hydrophobic binding pocket of MD-2 form a hydrophobic surface that interacts with Phe-440 and Phe-463 on a neighboring TLR4.MD-2.LPS complex, driving TLR4 activation." @default.
• W2049027654 created "2016-06-24" @default.
• W2049027654 creator A5019466019 @default.
• W2049027654 creator A5023095988 @default.
• W2049027654 creator A5025133435 @default.
• W2049027654 creator A5053304059 @default.
• W2049027654 creator A5055222513 @default.
• W2049027654 creator A5077520160 @default.
• W2049027654 creator A5090064644 @default.
• W2049027654 date "2009-05-01" @default.
• W2049027654 modified "2023-10-18" @default.
• W2049027654 title "Essential Roles of Hydrophobic Residues in Both MD-2 and Toll-like Receptor 4 in Activation by Endotoxin" @default.
• W2049027654 cites W127412881 @default.
• W2049027654 cites W1483162601 @default.
• W2049027654 cites W1496461152 @default.
• W2049027654 cites W1532387244 @default.
• W2049027654 cites W1585311024 @default.
• W2049027654 cites W1590494234 @default.
• W2049027654 cites W1597676074 @default.
• W2049027654 cites W1872584468 @default.
• W2049027654 cites W1963869935 @default.
• W2049027654 cites W1970216382 @default.
• W2049027654 cites W1974167823 @default.
• W2049027654 cites W1979359195 @default.
• W2049027654 cites W1980892269 @default.
• W2049027654 cites W1991412427 @default.
• W2049027654 cites W1992245975 @default.
• W2049027654 cites W1995017064 @default.
• W2049027654 cites W2017421343 @default.
• W2049027654 cites W2029157767 @default.
• W2049027654 cites W2030728145 @default.
• W2049027654 cites W2034222489 @default.
• W2049027654 cites W2036048288 @default.
• W2049027654 cites W2068365384 @default.
• W2049027654 cites W2069556266 @default.
• W2049027654 cites W2070416862 @default.
• W2049027654 cites W2078337496 @default.
• W2049027654 cites W2078895028 @default.
• W2049027654 cites W2086676026 @default.
• W2049027654 cites W2091628997 @default.
• W2049027654 cites W2093067468 @default.
• W2049027654 cites W2095833996 @default.
• W2049027654 cites W2096671043 @default.
• W2049027654 cites W2110768898 @default.
• W2049027654 cites W2111017378 @default.
• W2049027654 cites W2113532250 @default.
• W2049027654 cites W2129319296 @default.
• W2049027654 cites W2130655121 @default.
• W2049027654 cites W2130840482 @default.
• W2049027654 cites W2139147781 @default.
• W2049027654 cites W2150736048 @default.
• W2049027654 cites W2153983456 @default.
• W2049027654 cites W2164094601 @default.
• W2049027654 cites W2166722407 @default.
• W2049027654 doi "https://doi.org/10.1074/jbc.m901429200" @default.
• W2049027654 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2685687" @default.
• W2049027654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19321453" @default.
• W2049027654 hasPublicationYear "2009" @default.
• W2049027654 type Work @default.
• W2049027654 sameAs 2049027654 @default.
• W2049027654 citedByCount "101" @default.
• W2049027654 countsByYear W20490276542012 @default.
• W2049027654 countsByYear W20490276542013 @default.
• W2049027654 countsByYear W20490276542014 @default.
• W2049027654 countsByYear W20490276542015 @default.
• W2049027654 countsByYear W20490276542016 @default.
• W2049027654 countsByYear W20490276542017 @default.
• W2049027654 countsByYear W20490276542018 @default.
• W2049027654 countsByYear W20490276542019 @default.
• W2049027654 countsByYear W20490276542020 @default.
• W2049027654 countsByYear W20490276542021 @default.
• W2049027654 countsByYear W20490276542022 @default.
• W2049027654 countsByYear W20490276542023 @default.
• W2049027654 crossrefType "journal-article" @default.
• W2049027654 hasAuthorship W2049027654A5019466019 @default.
• W2049027654 hasAuthorship W2049027654A5023095988 @default.
• W2049027654 hasAuthorship W2049027654A5025133435 @default.
• W2049027654 hasAuthorship W2049027654A5053304059 @default.
• W2049027654 hasAuthorship W2049027654A5055222513 @default.
• W2049027654 hasAuthorship W2049027654A5077520160 @default.
• W2049027654 hasAuthorship W2049027654A5090064644 @default.
• W2049027654 hasBestOaLocation W20490276541 @default.
• W2049027654 hasConcept C1009742 @default.
• W2049027654 hasConcept C104317684 @default.
• W2049027654 hasConcept C12554922 @default.
• W2049027654 hasConcept C134018914 @default.
• W2049027654 hasConcept C143065580 @default.
• W2049027654 hasConcept C144464004 @default.
• W2049027654 hasConcept C16318435 @default.
• W2049027654 hasConcept C170493617 @default.
• W2049027654 hasConcept C185592680 @default.
• W2049027654 hasConcept C2776070231 @default.
• W2049027654 hasConcept C2778754761 @default.
• W2049027654 hasConcept C55493867 @default.
• W2049027654 hasConcept C71240020 @default.
• W2049027654 hasConcept C86803240 @default.
• W2049027654 hasConceptScore W2049027654C1009742 @default.
• W2049027654 hasConceptScore W2049027654C104317684 @default.

• next