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- W2049062013 abstract "The coupling of enzymes with sol–gel technology creates excitingpossibilities for biosensing. Enzymes can be highly selective and willonly respond to specific analytes. Sol–gels provide a unique matrixin which various biomaterials can be immobilized without any loss ofenzyme activity. These two components have been combined for the opticalbiosensing of nitrate ions. The periplasmic nitrate reductase (Nap)extracted from the denitrifying bacterium Thiosphaera pantotrophareacts specifically with the nitrate (NO 3 -)anion. The encapsulation of this enzyme in a sol–gel structure forthe optical biosensing of nitrate ions is reported. The reduction ofnitrate by periplasmic nitrate reductase results in a characteristicchange in the UV/VIS absorption spectrum of the nitrate reductase. Thisspectroscopic change has been quantitatively calibrated against nitrateconcentration. The nitrate biosensing system is fully reversible and ishighly sensitive and selective to nitrate ions. The results obtained showthat the activity of the enzyme is not affected by the sol–gelmatrix, even after a storage period of up to six months. As no leaching ofthe Nap from the sol–gel matrix was observed, it is clear that theencapsulation of this nitrate sensitive enzyme in a sol–gel mediumrepresents an ideal anionic recognition element of an optical biosensorfor the detection of nitrate ions in the µmoll - 1 range." @default.
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- W2049062013 date "1997-01-01" @default.
- W2049062013 modified "2023-10-18" @default.
- W2049062013 title "Optical Biosensing of Nitrate Ions Using a Sol–Gel Immobilized Nitrate Reductase" @default.
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- W2049062013 doi "https://doi.org/10.1039/a606146j" @default.
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